红花玉兰硬枝扦插生根解剖结构及酶生理响应研究  

Study on rooting anatomical structure and enzyme physiological response of hardwood cuttings of Magnolia wufengensis

作　　者：梁玉婷 夏文胜 王朴 段庆明 LIANG Yu-ting;XIA Wen-sheng;WAGN Pu;DUAN Qing-ming(Wuhan Institute of Landscape Architecture,Wuhan 430081,China)

机构地区：[1]武汉市园林科学研究院,武汉430081

出　　处：《湖北农业科学》2021年第20期94-99,共6页Hubei Agricultural Sciences

摘　　要：以当年生红花玉兰(Magnolia wufengensis L.Y.Ma et L.R.Wang)半木质化枝条为材料,通过石蜡切片法对插穗生根部位进行解剖学观察并测定扦插枝条过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)活性,研究750 mg/L NAA诱导红花玉兰不定根产生的过程。结果表明,红花玉兰扦插枝条在生根过程中清水处理组(CK)和生长调节剂处理组POD活力均呈增加趋势,直至35 d时含量达到最高;PPO活力均呈先增加后降低的趋势,生长调节剂处理组和CK组在0~14 d时呈上升趋势,14 d后急剧下降;而IAAO活力则具有不同的趋势,具体表现为0~7 d CK组先下降,随后逐渐上升。解剖结构显示,扦插前的红花玉兰插穗内无潜伏根原基存在,不定根由扦插后形成的诱生根原基发育成,其中皮部产生的不定根起源于木质部和韧皮部之间维管形成层,愈伤产生的不定根是由愈伤组织形成的木质部特化而成。红花玉兰属于难生根的类型;低活性PPO、POD、内生高活性IAAO有利于生根,需要额外补施生长调节剂以促进生根;推测红花玉兰可能为诱导生根型树种,不定根产生方式有皮部生根和愈伤组织生根两种。The semi-lignified branches of Magnolia wufengensis were used as materials.The anatomical observation of rooting parts of cuttings was carried out by paraffin section method,and the activities of peroxidase(POD),polyphenol oxidase(PPO)and indoleace⁃tic acid oxidase(IAAO)in cutting branches were determined,and the process of adventitious root formation induced by 750 mg/L NAA was studied.The results showed that during the rooting process,the POD activity in the water treatment group(CK)and the growth regulator treatment group showed an increasing trend,and the content reached the highest at 35 d.The activity of PPO in⁃creased first and then decreased.The activity of PPO in the growth regulator treatment group and CK group increased from 0 to 14 d,and decreased sharply after 14 d.The activity of IAAO showed different trends.The activity of IAAO decreased first and then increased gradually in CK group from 0 to 7 d.The anatomical structure showed that there was no latent root primordium in the cuttings,cortex rooting type was developed from the inducted root primordium formed after cutting.Callus rooting type was originated from the vascular cambium between xylem and phloem.Adventitious roots produced by callus were specialized xylem formed by callus.Magnolia wufen⁃gensis belongs to the type of difficult to root.Low activity PPO,POD and high activity IAAO are beneficial to rooting,and additional growth regulators are needed to promote rooting.It is speculated that Magnolia wufengensis may be an induced rooting tree species,and adventitious roots can be generated by skin rooting and callus rooting.

关 键 词：红花玉兰(Magnolia wufengensis L.Y.Ma et L.R.Wang) 扦插 解剖结构 酶活性 生理响应 

分 类 号：S685.15[农业科学—观赏园艺]