miR-221和TIMP2在早发型重度子痫前期合并胎儿生长受限患者血清和胎盘中的表达  

Expression of microRNA-221 and matrix metalloproteinase inhibitor 2 in serum and placenta tissue in women with early-onset severe preeclampsia complicated with fetal growth restriction

作　　者：陈嵘[1] 林小红[1] 黎云[1] 周宇恒[1] CHEN Rong;LIN Xiaohong;LI Yun;ZHOU Yuheng(Guangdong Maternal and Child Health Care Hospital, Guangdong Province, 511400)

机构地区：[1]广东省妇幼保健院,广州511400

出　　处：《中国计划生育学杂志》2021年第8期1707-1710,共4页Chinese Journal of Family Planning

基　　金：广东省中医药局科研项目(20212026)。

摘　　要：目的:探讨微小RNA-221(miR-221)、基质金属蛋白酶抑制因子2(TIMP-2)在早发型重度子痫前期(EOSP)合并胎儿生长受限(FGR)患者血清和胎盘中的表达及临床意义。方法:选择2018年9月-2020年10月在本院就诊的EOSP患者256例,根据FGR将患者分为非FGR组139例和FGR组117例。酶联免疫吸附法检测两组血清TIMP-2水平,qRT-PCR检测血清与胎盘组织中miR-221表达,免疫组化法检测胎盘组织TIMP-2蛋白表达,Pearson法分析血清miR-221、TIMP-2表达水平与新生儿体重相关性,ROC曲线分析miR-221、TIMP-2对EOSP患者发生FGR的预测价值。结果:与非FGR组比较,FGR组血清与胎盘组织中miR-221(1.65±0.23、2.32±0.21)、血清TIMP-2(38.52±8.36 ng/ml)升高,胎盘组织TIMP-2蛋白阳性表达率(82.1%)升高(P<0.05);相关性分析显示,miR-221、TIMP-2表达水平均与新生儿体重呈负相关性(P<0.05);血清miR-221、TIMP-2联合预测EOSP患者发生FGR曲线下面积为0.921,敏感度88.9%,特异度84.2%。结论:EOSP合并FGR患者血清和胎盘中miR-221、TIMP-2高表达,且与胎儿生长受限有关,血清miR-221、TIMP-2有望成为评估EOSP发生FGR的分子标志物。Objective:To investigate the expression and clinical significance of microRNA-221(miR-221)and matrix metalloproteinase inhibitor 2(TIMP-2)in serum and placenta tissue of pregnant women with early-onset severe preeclampsia(EOSP)and fetal growth restriction(FGR).Methods:256 pregnant women with EOSP were selected as the observation objects from September 2018 to October 2020.According to the FGR situation,these women were divided into group A(139 women without FGR)and group B(117 women without FGR).Enzyme-linked immunosorbent assay was used to detect the serum TIMP-2 level of the women in the two groups.qRT-PCR was used to detect the expression of miR-221 in serum and placenta tissue.Immunohistochemical method was used to detect TIMP-2 protein expression in placenta tissue.Pearson method was used to analyze the correlation between the serum miR-221,TIMP-2 expression levels of the women and their newborn weight,and ROC curve was used to analyze the predictive value of miR-221 and TIMP-2 levels for FGR of the women with EOSP.Results:The levels of MiR-221 in serum and placental tissue(1.65±0.23 and 2.32±0.21),the serum TIMP-2 level(38.52±8.36 ng/ml),and the positive expression rate of TIMP-2 protein in placental tissue(82.1%)of the women in group B were significant higher than those of the women in group A(P<0.05).Correlation analysis showed that miR-221 and TIMP-2 expression levels of the women were negatively correlated with their neonatal weight(P<0.05).The area under the curve of the combined levels of serum miR-221 and TIMP-2 for predicting FGR occurrence of the women with EOSP was 0.921,and which’s sensitivity and specificity were 88.9%and 84.2%.Conclusion:The expressions of serum miR-221 and TIMP-2 in the serum and placenta of the pregnant women with EOSP and FGR are high,and which is related to their FGR occurrence.So the serum miR-221 and TIMP-2 levels are expected to be molecular markers for evaluating FGR of the women with EOSP.

关 键 词：早发型重度子痫前期 胎儿生长受限 微小RNA-221 基质金属蛋白酶抑制因子2 血清 胎盘 预测 

分 类 号：R714.5[医药卫生—妇产科学] R714.244[医药卫生—临床医学]