LC-MS分析穿心莲内酯的降解产物  

Analysis on the Degradation Products of Andrographolidume by LC-MS

作　　者：许静 彭红[2] 熊贤招 XU Jing;PENG Hong;XIONG Xian-zhao(Fuyang Hospital Affiliated to Anhui Medical University,Fuyang 236000,China;Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]安徽医科大学附属阜阳医院,安徽阜阳236000 [2]江西中医药大学,南昌330004

出　　处：《江西中医药大学学报》2021年第5期83-87,共5页Journal of Jiangxi University of Chinese Medicine

摘　　要：通过LC-MS分析穿心莲内酯的降解产物。液相条件:色谱柱:Aglient ZORBAX Eclipse Plus C18色谱柱(2.1 mm×100 mm,1.8μm);流动相:乙腈-水,梯度洗脱;流速:0.2 mL·min-1;柱温:25℃;检测波长为230 nm;进样量:2 μL。质谱条件离子源:电喷雾离子源;检出模式:正离子模式;扫描范围:m/z 501000。结果显示穿心莲内酯与强制破坏试验产生的降解产物分离良好,初步鉴定了11种杂质。LC-MS was used to detect the degradation products of andrographolidume.The HPLC system consisted of an Aglient ZORBAX Eclipse Plus C18(2.1 mm × 100 mm,1.8 μm) by liner gradient elution using acetonitrilel-water and the flow rate was 0.2 mL·min-1.The column temperature was 25 C.The detection wavelength was 230 nm and injection volme was 2 μL.The positive ion mode was detected by electrospray ionization.The detection ions were within the range from m/z 50 to m/zl 000.Results show that andrographolidume was well separated from degradation products produced by forced destruction.Eleven kinds of impurities were identified from.

关 键 词：穿心莲内酯 降解产物 LC-MS 

分 类 号：R284[医药卫生—中药学]