水凝胶对腹腔开放下小肠氧化应激的保护作用  

Protective effect of hydrogel on oxidative stress in small intestine after open abdomen

作　　者：周翔[1] 赵泽玉 郭正昌 李大路 张宗耀[1] 周波[1] 常家聪[1] Zhou Xiang;Zhao Zeyu;Guo Zhengchang(Dept of General Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2021年第10期1580-1584,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81801592);安徽省自然科学基金(编号:1708085QH199)。

摘　　要：目的探究水凝胶对腹腔开放下大鼠小肠氧化应激的保护作用。方法用过量的对硝基苯氯甲酸酯(PNC)活化聚乙二醇(PEG)两端的羟基,得到PNC-PEG-PNC。用酪胺和壳聚糖分别置换PNC-PEG-PNC分子两侧的PNC,得到壳聚糖-PEG-酪胺分子(CPT)。对60只大鼠建立腹腔间隔室综合征模型,后将其随机分为水凝胶组(CPT组)、暂时性关腹组(TAC组)、假手术组(Sham组)。CPT组:腹腔开放后,以生物材料CPT水凝胶被覆于开放创面上,再用聚丙烯网片临时关腹;TAC组:腹腔开放后用聚丙烯网片临时关腹;Sham组:逐层进腹后随即关腹。腹腔开放后4 h,使用免疫组织荧光染色检测CPT组和TAC组大鼠小肠上皮的氧化应激损伤细胞数量。腹腔开放后4、8、24 h分别使用TBA法和ELISA法测定CPT组、TAC组和Sham组小肠组织的丙二醛(MDA)和8-羟脱氧鸟苷(8-OHDG)水平。结果腹腔开放后4 h,CPT组未观察到肠上皮细胞氧化应激损伤现象,而TAC组可以观察到损伤细胞。腹腔开放后4 h,TAC组的MDA和8-OHDG水平明显高于其在Sham组的水平,CPT组的前述指标浓度在TAC组和Sham组之间。在腹腔开放后8 h,CPT组大鼠小肠MDA和8-OHDG的浓度恢复至假手术组水平。在关腹后8 h和24h,TAC组的MDA和8-OHDG的浓度有所降低,但仍高于假手术组。结论生物材料CPT水凝胶能显著降低腹腔开放后小肠氧化应激水平并有利于保护小肠损伤。Objective To investigate the effect of hydrogel on oxidative stress and the protective effect of intestinal injury in rats after open abdomen.Methods A poly(ethylene glycol)modified with tyramine was grafted onto a chitosan backbone to enhance the solubility of the chitosan and to crosslink into three-dimensional networks.Followed by intraperitoneal injection of air to induce abdominal compartment syndrome,sixty rats were randomly divided into hydrogel group(CPT group),temporary abdominal closure group(TAC group)and sham operation group(Sham group).Hydrogel group was treated with chitosan-polyethylene-tyramine molecular hydrogel coated on the intestine,and then closed with a polypropylene mesh after open abdomen(CPT group).Temporary abdominal closure group was temporarily closed with a polypropylene mesh after open abdomen(TAC group).Sham group was undergone laparotomies and then closed immediately(Sham group).At immediately four hours after open abdomen,the oxidative stress cells in the small intestinal epithelium of the hydrogel group and the temporary abdominal closure group were detected by immunohistofluorescence.The levels of malondialdehyde(MDA)and 8-OHDG in intestinal homogenate of the three groups were measured at specific time points by TBA and ELISA respectively.Results At 4 h after open abdomen,no oxidative stress injury was observed in intestinal epithelial cells in the CPT group and oxidative stress injury of intestinal epithelial cells was observed in the TAC group.Four hours after open abdomen,MDA and 8-OHDG levels were significantly highest in the TAC group,followed by CPT group,with Sham group was the least.At 8 h and 24 h after open abdomen,MDA and 8-OHDG levels in CPT group returned to Sham values,whereas these levels in TAC group were higher than those of Sham group.Conclusion The biomaterial chitosan-polyethylene glycol-tyramine molecular hydrogels can significantly reduce oxidative stress levels and protect against intestinal damage in small intestine after open abdomen.

关 键 词：腹腔开放 氧化应激 小肠损伤 水凝胶 

分 类 号：R656.7[医药卫生—外科学]