原发性肝癌组织中PKM2、COX2及IAPs的表达及其与放疗敏感性的关系分析  

Expression of PKM2,COX2 and IAPs in cancer tissues of patients with primary liver cancer and its relationship with radiotherapy sensitivity

作　　者：徐明静 芦东徽 高世乐 马欢 方向[2] 钱立庭 Xu Mingjing;Lu Donghui;Gao Shile(Dept of Oncology,The Affiliated Provincial Hospital of Anhui Medical University,Hefei 230000;Dept of Oncology,The East District of the First Affiliated Hospital of Anhui Medical University(Feidong County People′s Hospital),Hefei 231600)

机构地区：[1]安徽医科大学附属省立医院肿瘤内科,合肥230000 [2]安徽医科大学第一附属医院东区(肥东县人民医院)肿瘤内科,合肥231600

出　　处：《安徽医科大学学报》2021年第10期1612-1616,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1808085MH239)。

摘　　要：目的探究原发性肝癌患者癌组织中M2型丙酮酸激酶(PKM2)、环氧合酶2(COX2)及凋亡蛋白抑制因子(IAPs)的表达及其与放疗敏感性的关系。方法选取接受三维适形放疗的原发性肝癌患者105例,经肝脏穿刺活检采集肝癌组织,采用免疫组化法检测肝癌组织中PKM2、COX2及IAPs家族[X关联凋亡抑制因子(XIAP)、细胞内凋亡抑制蛋白1(cIAP-1)]蛋白的表达,比较不同放疗效果和敏感性患者癌组织中PKM2、COX2、XIAP及cIAP-1蛋白表达的差异,并分析上述蛋白表达与患者预后的关系。结果105例患者肝癌组织中PKM2、COX2、XIAP及cIAP-1蛋白表达阳性率分别为81.90%、74.28%、85.71%及82.86%;COX2、XIAP蛋白阳性表达者治疗有效率与阴性者比较,差异有统计学意义(P<0.05);放疗敏感患者临床分期、分化程度、肿瘤直径、肝癌组织中PKM2、COX2、XIAP及cIAP-1蛋白表达情况与放疗不敏感者比较,差异有统计学意义(P<0.05);高临床分期、低分化程度、COX2、XIAP阳性表达是患者放疗不敏感的独立危险因素(P<0.05);XIAP阳性表达者复发率和转移率与阴性表达者比较,差异有统计学意义(P<0.05)。结论原发性肝癌患者癌组织中PKM2、COX2、XIAP及cIAP-1阳性率高,其中COX2、XIAP蛋白表达与患者放疗效果、敏感性密切相关,XIAP蛋白表达还与患者复发转移有关。Objective To explore the expression of M2 pyruvate kinase(PKM2),cyclooxygenase 2(COX2)and inhibitor of apoptosis protein(IAPs)in cancer tissues of patients with primary liver cancer(PLC)and its relationship with radiotherapy sensitivity.Methods A total of 105 PLC patients who underwent three-dimensional conformal radiation therapy(CRT)were enrolled.Liver cancer tissues were collected by liver needle biopsy.The expression of PKM2,COX2 and IAPs protein families[X-linked inhibitor of apoptosis protein(XIAP),cellular inhibitor of apoptosis protein 1(cIAP-1)]in liver cancer tissues was detected by immunohistochemistry.The differences in expression of PKM2,COX2,XIAP and cIAP-1 in cancer tissues among patients with different radiotherapy effects and sensitivity were compared.The relationship between the expression of above-mentioned proteins and prognosis was analyzed.Results The positive expression rates of PKM2,COX2,XIAP and cIAP-1 in liver cancer tissues of the 105 patients were 81.90%,74.28%,85.71%and 82.86%,respectively.There were significant differences in response rate of treatment between patients with positive expression of COX2 and XIAP and those with negative expression(P<0.05).There were significant differences in clinical staging,differentiation degree,tumor diameter,expression of PKM2,COX2,XIAP and cIAP-1 in liver cancer tissues between patients with radiotherapy sensitivity and those without radiotherapy sensitivity(P<0.05).High clinical staging,poor differentiation,positive expression of COX2 and XIAP were independent risk factors of radiotherapy insensitivity(P<0.05).There were significant differences in recurrence rate and metastasis rate between patients with positive expression of XIAP and those with negative expression(P<0.05).Conclusion The positive rates of PKM2,COX2,XIAP and cIAP-1 are high in liver cancer tissues of PLC patients.The expression of COX2 and XIAP is closely related to radiotherapy effect and sensitivity,and the expression of XIAP is also related to recurrence and metastasis.

关 键 词：原发性肝癌 M2型丙酮酸激酶 环氧和酶2 凋亡抑制因子 放疗敏感性 预后 

分 类 号：R246.5[医药卫生—针灸推拿学]