产丙酮酸棒状杆菌肽聚糖的提取和抗血流感染作用的研究  

Extraction of the peptidoglycan of Corynebacterium pyruviciproducens and the study on the effects of anti-bloodstream infection

作　　者：章杨 周强[1] 陈礼文[1] 刘周[1] 韩清珍[2] 史进方[2] Zhang Yang;Zhou Qiang;Chen Liwen(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601 [2]苏州大学附属第一医院临床检测中心,苏州215006

出　　处：《安徽医科大学学报》2021年第10期1665-1669,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1908085QH366)。

摘　　要：采用三氯乙酸法提取产丙酮酸棒状杆菌肽聚糖(CP-PGN),经溶菌酶溶解试验对提取终产物的纯度进行验证。并分析该产物的分子量、结构、氨基酸的组成。通过构建多重耐药的肺炎克雷伯菌(KPN)及白色念珠菌(CAL)的血流感染的动物模型,评价CP-PGN抗血流感染的效果。通过溶菌酶溶解试验可得,提取终产物为肽聚糖,分子量为18774 u,傅里叶红外光谱分析显示存在肽聚糖的特征峰,采用国家标准GB/T14965-1994对其氨基酸的种类进行了检测。在动物实验中,CP-PGN能有效延长小鼠的生存时间,提高生存率,发挥不同程度的预防作用。所提取的CP-PGN对KPN及CAL引起的血流感染具有显著预防与抵抗作用。Extraction of CP-PGN was processed according to the TCA method,the purity of the final product was verified by treating with the lysozyme.Then the molecular weight,structures and compositions of amino acids of CP-PGN were studied.The animal models of bloodstream infection were established by using carbapenenes producing Klebsiella pneumonia(KPN)and Candida albicans(CAL)to the evaluation of the effect of CP-PGN against bloodstream infection.The lysozyme dissolution experiment verified that the extracted product was peptidoglycan and CP-PGN possessed the characteristics of peptidoglycan.The molecular weight of CP-PGN is 18774 u,which showed the characteristic peaks of PGN by infrared spectroscopy.The GB/T14965-1994 was used to detect its compositions of amino acids.CP-PGN significantly prolonged the survival time,improved the survival rate of mice and showed different degree of prevention in the animal experiments.All these showed that the extracted product of CP-PGN had the significant preventive and resistant effects on KPN and CAL-induced bloodstream infection.

关 键 词：产丙酮酸棒状杆菌肽聚糖 血流感染 多重耐药的肺炎克雷伯菌 白色念珠菌 

分 类 号：R379.4[医药卫生—病原生物学] R378.996[医药卫生—基础医学]