云南省部分地区鸡住白细胞虫的分子鉴定与流行分析  

Molecular Identification and Epidemic Analysis of Leucocytozoon in Chicken in some Areas of Yunnan Province

作　　者：董仙兰 翟国莲 徐聪 杜娟娟 普丽花 王开胜 DONG Xian-lan;ZHAI Guo-lian;XU Cong;DU Juan-juan;PU Li-hua;WANG Kai-sheng(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;Animal Health Inspection Institute in Yongde,Lincang 677600,China;Yuxi Animal Disease Control Center,Yuxi 653100,China;College of Biology and Pharmacy,Yulin Normal University,Yulin 537000,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]云南省永德县动物卫生监督所,云南临沧677600 [3]玉溪市动物疫病预防控制中心,云南玉溪653100 [4]玉林师范学院生物与制药学院,广西玉林537000

出　　处：《中国兽医杂志》2021年第6期47-52,共6页Chinese Journal of Veterinary Medicine

基　　金：云南省专家工作站项目(202005AF150041);玉林师范学院高层次人才项目(G2019ZK47)。

摘　　要：为明确云南地区部分养鸡场住白细胞虫的流行情况,试验采用巢式PCR方法对970份鸡血样扩增住白细胞虫的线粒体细胞色素b基因(cytb)。结果显示:34份样本为住白细胞虫阳性,阳性检出率为3.51%(34/970);同源性分析表明,阳性样本均为卡氏住白细胞虫(Leucocytozoon caulleryi)。对阳性样本进行不同性别、日龄、品种和地区等因素比对分析,发现地理区域、日龄、品种间的阳性率均存在极显著相关性(P<0.001),而性别间无显著相关性(P>0.05)。本试验结果可为云南养鸡场住白细胞虫病的防治提供参考。In order to understand the prevalence of Leucocytozoon in some chicken farms in Yunnan province, the mitochondrial cytochrome b gene(cytb) of Leucocytozoon was amplified from 970 chicken blood samples by nested PCR. The results showed that 34 samples were positive for Leucocytozoon,and the positive detection rate was 3.51%(34/970). Homology analysis showed that the positive samples were all Leucocytozoon caulleryi. The positive samples were analyzed by gender, age, breed and region, and there was a significant correlation among geographic area, age and breed(P<0.001),and there was no significant correlation between genders(P>0.05). The results of this study can provides reference for the prevention and control of Leucocytozoonosis in chicken farms in Yunnan province.

关 键 词：云南 住白细胞虫 分子流行病学调查 线粒体细胞色素B基因 

分 类 号：S855.9[农业科学—临床兽医学]