RBP4在成骨机制中的蛋白组学研究  

Study on proteomics of RBP4 in osteogenesis

作　　者：Abdulsamad Ghanem 柯荣湖 穆雄铮 吴颖之 Abdulsamad Ghanem;KE Ronghu;MU Xiongzheng;WU Yingzhi(Department of Plastic Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区：[1]复旦大学附属华山医院整形外科,上海市200040

出　　处：《组织工程与重建外科》2021年第5期378-382,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：上海市卫生健康委员会青年课题(20174Y0088)。

摘　　要：目的对分化阶段中过表达RBP4的MC3T3⁃E1细胞进行定量蛋白组学分析,为后续研究颅缝早闭发病机制中异常成骨的研究奠定基础。方法使用LENTI⁃RBP4转染小鼠MC3T3⁃E1成骨细胞,使其过表达RBP4;培养收集传代细胞,提取蛋白,进行质谱分析,并通过Western blot实验来验证蛋白组学的结果。结果过表达RBP4小鼠成骨细胞中的差异蛋白主要集中在PI3K⁃AKT信号通路和MAPK信号通路。经Western blot验证,RBP4过表达组的Ikbkg、Akt和Col1a1表达量显著高于空载体组,而Nfkb1、Traf2、Cdk4、RAS、Mapk3和Braf表达量则明显下调。结论过表达RBP4可能通过同时激活PI3K信号通路和拮抗MAPK信号通路来促进成骨细胞分化。Objective To perform quantitative proteomics analysis on RBP4⁃overexpressed MC3T3⁃E1 cells in the differentiation stage,and to lay a foundation for the subsequent study of abnormal osteogenesis in the pathogenesis of craniosynostosis.Methods Mouse MC3T3⁃E1 osteoblasts were transfected with LENTI⁃RBP4 to over⁃express RBP4,passaged cells were cultured and collected for protein extraction and mass spectrometry analysis.Western blot was used to verify the proteomics result.Results The protein expression of PI3K⁃AKT,MAPK signaling pathways in RBP4 overexpression group were significantly different.Western blot verified that the expression levels of Ikbkg,Akt and Col1a1 in RBP4 overexpression group were significantly increased compared with empty vector group,while the expression levels of Nfkb1,Traf2,Cdk4,RAS,Mapk3 and Braf were significantly down⁃regulated.Conclusion The overexpression RBP4 might promote the osteoblast differentiation by activating PI3K⁃AKT signaling pathway and inhibiting MAPK signaling pathway.

关 键 词：小鼠成骨细胞 视黄醇结合蛋白4 基因本体分析 通路分析 

分 类 号：R682.1[医药卫生—骨科学]