黄芪多糖和炙黄芪多糖调节大鼠肠道菌群组成与多样性的比较研究  

Comparative Study of Astragalus Polysaccharides and Honey-Processed Astragalus Polysaccharides on Composition and Diversity of Gut Microbiota in Rats

作　　者：吴莉[1] 舒青龙[1] 刘玉琼 唐芳瑞[1] Wu Li;Shu Qinglong;Liu Yuqiong;Tang Fangrui(College of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004)

机构地区：[1]江西中医药大学中医学院,南昌330004

出　　处：《中药药理与临床》2021年第4期40-47,共8页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省卫生和计划生育委员会(编号:2018A342);江西省“双一流”学科(中医学)建设资助项目(编号:JXSYLXK-ZHYI054,JXSYLXK-ZHYI051)。

摘　　要：目的:比较研究黄芪多糖和炙黄芪多糖对抗生素相关性腹泻大鼠肠道微生态平衡的调节作用。方法:依照蜜炙法炮制黄芪得炙黄芪,并用水提醇沉法得到黄芪多糖和炙黄芪多糖。50只大鼠,随机选取其中40只以盐酸林可霉素注射液10 mL/kg每天两次,连续灌胃3 d,当出现便溏,体重减轻等症状时,说明造模成功,再分为模型对照组、自然恢复组、黄芪多糖400 mg/kg组和炙黄芪多糖400 mg/kg组,剩余10只作为空白对照组。观察记录大鼠的腹泻情况及体重变化,用HE染色观察肠道结构完整性,用16S-rRNA高通量测序技术对大鼠粪便肠道菌群进行基因测序。结果:与正常对照组比,模型对照组大鼠出现大便稀溏,结肠有一定的形态学损伤;在门水平上,拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)占比显著增加,厚壁菌门(Firmicutes)占比显著减少(P<0.01);在属水平上,拟杆菌(Bacteroides)占比显著增加,乳酸菌(Lactobacillus)、罗斯氏菌(Robeburia)和unidentifiedRuminococcaceae占比减少(P<0.05或P<0.01)。与自然恢复组比,黄芪多糖400 mg/kg组和炙黄芪多糖400 mg/kg组均能缓解大鼠的腹泻情况,结肠组织的形态学损伤也得到改善;在门水平上,两种多糖组的拟杆菌门和变形菌门占比显著减少,厚壁菌门占比明显增加(P<0.05);在属水平上,炙黄芪多糖400 mg/kg组能显著增加3种有益菌(乳酸菌、罗斯氏菌和unidentifiedRuminococcaceae)的占比(P<0.05或P<0.01),而黄芪多糖400 mg/kg组能显著增加拟杆菌和有益菌乳酸菌的占比(P<0.01)。结论:在调节抗生素相关性腹泻大鼠肠道菌群丰富度和多样性方面,炙黄芪多糖效果优于黄芪多糖。Objective:To compare the effects of astragalus polysaccharides(APS) and honey-processed astragalus polysaccharides(HAPS) on the regulation of the composition and diversity of the gut microbiota in antibiotic-associated diarrhea(AAD) rats. Methods:APS and HAPS were obtained by water extraction and alcohol precipitation from Astragali Radix and honey-roasted Astragali Radix. Fifty rats were randomly selected and 10 were assigned to the normal control group. The remaining 40 rats were orally administered with lincomycin hydrochloride(10 mL/kg) twice a day for 3 consecutive days. When symptoms such as loose stools and weight loss were observed, the AAD model was established. The model rats were divided into a model group, a natural recovery group, an APS(400 mg/kg) group, and a HAPS(400 mg/kg) group. The rat diarrhea and body weight changes were observed. The structural integrity of the intestines was observed by HE staining, and 16 S-rRNA high throughput sequencing technology was used to perform gene sequencing on the gut microbiota of rat feces. Results: Compared with the normal control group, the model group showed loose stools and certain morphological damage in the colon. At the phylum level, the proportions of Bacteroidetes and Proteobacteria increased, while the proportion of Firmicutes was reduced(P<0.01). At the genus level, the proportion of Bacteroides increased, while the proportions of Lactobacillus, Robeburia and unidentified_Ruminococcaceae decreased(P<0.05 or P<0. 01). Compared with the natural recovery group, both the APS group and the HAPS group could alleviate the diarrhea of the rats and the morphological damage in colon tissues. At the phylum level, the proportions of Bacteroidetes and Proteobacteria in the APS group and the HAPS group were reduced, while the proportion of Firmicutes was elevated(P<0.05). At the genus level, the HAPS group increased the proportions of three types of probiotics(Lactobacillus, Robeburia and unidentifiedRuminococcaceae)(P<0.05 or P<0.01), while the APS group only in

关 键 词：黄芪多糖 炙黄芪多糖 抗生素相关性腹泻 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]