蜘蛛香挥发油抑制小胶质细胞活化的机制研究  

Mechanism of Essential Oil from Valerianae Jatamansi Rhizoma et Radix in Inhibition of Microglia Activation

作　　者：杨南赟 曾凡利 张旭[1,2] 吴小盈 沈祥春[1,3] 龙庆德[1,2] Yang Nanyun;Zeng Fanli;Zhang Xu;Wu Xiaoying;Shen Xiangchun;Long Qingde(Guizhou Medical University,Guiyang 550025;Department of Medicinal Plants and Pharmacognosy in Guizhou Medical University,Guiyang 550025;Key Laboratory of Utilization of Natural Medicines in Guizhou Medical University,Guiyang 550025)

机构地区：[1]贵州医科大学,贵阳550025 [2]贵州医科大学药用植物与生药学教研室,贵阳550025 [3]贵州医科大学天然药物资源优效利用重点实验室,贵阳550025

出　　处：《中药药理与临床》2021年第4期79-83,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金喀斯特中心项目(编号:U1812403-4-4);贵州省高层次创新型人才百层次人才(贵州省科技厅黔科合人才[2015]4029号);贵州省特色天然药物资源活性研究科技创新团队(黔科合人才团队[2015]4025号);国家社科基金重大项目(编号:16ZDA238);贵州医科大学药学国际科技合作基地(黔科合平台人才[2017]5802号)。

摘　　要：目的:探讨蜘蛛香挥发油对脂多糖诱导小胶质细胞活化的抑制作用及可能机制。方法:建立脂多糖诱导小胶质细胞活化模型,并用不同剂量的蜘蛛香挥发油处理,MTT法检测细胞活力,ELISA法检测IL-6的分泌,实时荧光定量PCR检测Il-6、Il-1β、Nf-κb和Iκbα激酶的mRNA表达水平,Western Blot检测IL-6、IL-1β、NF-κB、IκBα激酶及其磷酸化蛋白的相对表达。结果:与正常对照组比较,模型对照组的IL-6含量显著升高(P<0.01),IL-6、IL-1β、核因子κB的蛋白表达以及mRNA表达显著上调(P<0.01),P-IκBα/IκBα的比值显著升高(P<0.05);与模型对照组比较,2、4μg/L蜘蛛香挥发油可显著降低小胶质细胞中IL-6的含量(P<0.05),1、2、4μg/L蜘蛛香挥发油均可不同程度下调IL-6、IL-1β、NF-κB的蛋白表达以及mRNA表达(P<0.05或P<0.01),上调Iκbα激酶的mRNA表达(P<0.05或P<0.01),并显著降低P-IκBα/IκBα的比值(P<0.01)。结论:蜘蛛香挥发油对LPS诱导的小胶质细胞活化具有抑制作用,其机制可能与抑制NF-κB/IκBα激酶信号通路有关。Objective:To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide(LPS). Methods: The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix. MTT assay was used to detect cell viability, ELISA to detect IL-6 secretion, RT-PCR to detect mRNA expression levels of IL-6, IL-1β, NF-κB, and IκBα,Western blotting to detect the protein expression of IL-6, IL-1β, NF-κB, IκBα, and their phosphorylated products. Results: Compared with the normal control group, the model group showed increased IL-6 content(P<0.01),up regulated mRNA and protein levels of IL-6, IL-1β, and NF-κB(P<0.01),and elevated ratio of P-IκBα/IκBα(P<0.05). Compared with the model group, 4 μg/L and 2 μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6(P<0.05), while 4 μg/L, 2 μg/L, and 1 μg/L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6, IL-1β, and NF-κB to varying degrees(P<0.05 or P<0.01), up-regulate the mRNA expression of IκBα(P<0.05 or P<0.01), and decreased the ratio of P-IκBα/IκBα(P<0.05 or P<0.01). Conclusion: Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation, and its mechanism may be related to the inhibition of the NF-κB/IκBα signaling pathway.

关 键 词：蜘蛛香挥发油 小胶质细胞 NF-κB/IκBα激酶信号通路 

分 类 号：R285.5[医药卫生—中药学]