两份大麦材料小孢子诱导愈伤及再生植株的倍性研究  

Study on the ploidy of microspore-derived calli and regenerated plants of two barley materials

作　　者：何婷[1,2] 郭桂梅 陆瑞菊[1,2] 陈志伟 李颖波[1,2] 徐红卫 周龙华[1,2] 黄琳丽 高润红[1,2] 刘成洪 HE Ting;GUO Guimeil;LU Ruiju;CHEN Zhiwei;LI Yingbo;XU Hongwei;ZHOU Longhua;HUANG Linli;GAO Runhong;LIU Chenghong(Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106

出　　处：《植物生理学报》2021年第8期1708-1714,共7页Plant Physiology Journal

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-2号];西藏自治区科技计划项目(XZ2019NA01-2020-016);现代农业产业技术体系建设专项资金(CARS-05-01A-02);上海市农业科学院农业科技创新支撑领域研究专项计划项目[(2017)B-01]。

摘　　要：利用流式细胞仪检测大麦(Hordeum vulgare)小孢子离体培养获得的愈伤组织和再生植株细胞核DNA倍性水平及其分布规律,结果表明:大麦‘花30’和‘甘啤1405’小孢子愈伤组织细胞在诱导培养第6天时已发生自发加倍,二倍体细胞数目随着诱导培养时间的延长表现出逐步增加趋势。后期分化时,单倍体、二倍体和四倍体再生植株所占比率分别为7.8%、85.5%、6.7%。两份材料的愈伤产量无明显差异,绿苗分化频率差异显著。结论:大麦小孢子愈伤组织细胞的自发加倍发生在多细胞结构形成(第6天)或更早时期;愈伤分化形成的不同倍性再生植株所占比率相对稳定,不受基因型影响。Flow cytometry was used to detect the ploidy level and distribution of nucleus DNA in calli and regenerated plantlets of barley(Hordeum vulgare)microspore culture in vitro.The results show that microspore-derived callus cells of barley‘Hua 30’and‘Ganpi 1405’doubled spontaneously at 6th day of induction,and the amount of doubled cells showed a gradual increasing trend with the extension of induction.The average percentages of haploid,diploid and tetraploid plantlets were 7.8%,85.5%and 6.7%,respectively,at the later stage of differentiation.There was no significant difference in callus yield between the two materials,but there was a significant difference in green plantlet differentiation frequency.In conclusion,the spontaneous doubling of barley microspore-derived callus cells occurs at the stage of multi-cellular structure formation(6th day)or earlier.The percentage of different ploidy-regenerated plantlets from calli differentiation was relatively stable,which was not affected by genotype.

关 键 词：大麦 小孢子 自发加倍 流式细胞仪 

分 类 号：S512.3[农业科学—作物学]