妊娠期高血压疾病易感lncRNA的筛选研究  

Screening of the susceptibility lncRNA in hypertensive disorders complicating pregnancy

作　　者：路兴军[1] 修霞[1] 齐峰[1] 徐健[1] 张胜利[1] Lu Xingjun;Xiu Xia;Qi Feng;Xu Jian;Zhang Shengli(Weifang Maternal and Child Health Hospital,Weifang Shandong,621011,China)

机构地区：[1]潍坊市妇幼保健院,山东潍坊261011

出　　处：《中外女性健康研究》2021年第17期4-5,24,共3页Women＇s Health Research

基　　金：山东省医药卫生科技发展计划项目(2018WSA07045)。

摘　　要：目的:筛选妊娠期高血压疾病(HDCP)胎盘组织中差异表达的长链非编码RNA(lncRNA),探讨lncRNA与HDCP的关系。方法:选择2018年5月至2019年5月本院收治的3例HDCP剖宫产患者为研究对象,同期3例正常妊娠剖宫产孕妇作为对照组。胎盘娩出后取母体面胎盘组织行RNA测序,筛选出差异表达的lncRNA和mRNA。结果:经RNA测序检测出差异表达的lncRNA共有19个,其中表达上调的有4个,表达下调的有15个;检测出差异表达的mRNA共有423个,其中表达上调的有84个,表达下调的有399个。结论:HDCP患者胎盘中存在大量差异表达的lncRNA和mRNA,可能与HDCP的发生有关。Objective:To Screen the susceptibility long non-coding RNA(lncRNA)in hypertensive disorders complicating pregnancy(HDCP).Methods:Three patients with HDCP who were treated in our hospital from May 2018 to May 2019 were selected as the research subjects and three healthy pregnant women who underwent a prenatal examination in the same hospital were selected as the control group.The placental tissues were taken from the patients.RNA-sequencing was performed to construct lncRNA/mRNA differential expression profiles.Results:RNA-seq analysis revealed 19 differentially expressed lncRNA(4 up-regulated;15 down-regulated)(P<0.05).Moreover,423 differentially expressed genes(DEGs)(84 up-regulated;339 down-regulated)(P<0.05).Conclusion:Differentially expressed lncRNAs in the placenta of patients with HDCP are detected,and these lncRNA may play a role in the occurrence and development of HDCP.

关 键 词：妊娠期高血压疾病 长链非编码RNA MRNA 胎盘组织 

分 类 号：R714.24[医药卫生—妇产科学]