高山姬鼠体重和产热特征在季节性模拟条件下的变化  

Changes in Body Mass and Thermogenesis Properties of Apodemus chevrieri Under Seasonal Simulation

作　　者：贾婷 朱万龙[2] Jia Ting;Zhu Wanlong(Yunnan Medical Health College,Kunming,Yunnan650106,China;School of Life Sciences,Yunnan Normal University,Kunming,Yunnan 650500,China)

机构地区：[1]云南医药健康职业学院,云南昆明650106 [2]云南师范大学生命科学学院,云南昆明650500

出　　处：《绿色科技》2021年第20期24-27,30,共5页Journal of Green Science and Technology

基　　金：云南省教育厅科学研究基金项目(编号:2021J1360);云南医药健康职业学院科学研究基金项目(编号:2020Y002)。

摘　　要：以横断山区的高山姬鼠为研究对象,将其分为夏季模拟组和冬季模拟组两个组,各驯化28 d,并测定了其体重、代谢率、摄食量、血清瘦素含量和下丘脑神经肽基因表达量等相关指标。结果表明:经过28 d驯化,高山姬鼠夏季模拟组的体重增加,驯化期间体重差异显著(F=3.21,P<0.05),冬季模拟组体重显著下降(F=2.98,P<0.05);夏季模拟组的摄食量变化差异不显著(P>0.05),冬季模拟组的摄食量极显著增加(F=5.89,P<0.01);夏季模拟组的RMR和NST变化差异不显著(P>0.05),冬季模拟组的RMR和NST显著增加(RMR:F=2.54,P<0.05;NST:F=3.56,P<0.05);夏季和冬季模拟组的血清瘦素含量变化差异显著(t=2.54,P<0.05),夏季模拟组高于冬季模拟组;夏季和冬季模拟组的下丘脑神经肽NPY和AgRP差异显著(NPY:F=-2.98,P<0.05;AgRP:F=-2.05,P<0.05),冬季模拟组的NPY和AgRP高于夏季模拟组。以上结果说明高山姬鼠主要通过降低体重、增加食物摄入量、提高代谢率以及增加下丘脑促食类神经肽基因表达量来适应类似冬季的寒冷条件。In the present study,Apodemus chevrieri in Hengduan mountain region was used as the research object,which was divided into two groups,the summer simulation group and the winter simulation group.Each group was acclimated for 28 days,and body mass,metabolic rate,food intake,serum leptin level and hypothalamic neuropeptide genes expression were measured.The results showed that after 28 days of acclimation,body mass of A.chevrieri in summer simulation group increased significantly(F=3.21,P<0.05),but body mass of simulation winter group decreased significantly(F=2.98,P<0.05).There was no significant difference in food intake in the simulation group in summer(P>0.05),which was significantly increased in winter simulation group(F=5.89,P<0.01).There was no significant difference in RMR and NST in the summer simulation group(P>0.05)while RMR and NST in the winter simulation group were significantly increased(RMR:F=2.54,P<0.05;NST:F=3.56,P<0.05).Serum leptin level showed significant differences between two simulated groups(t=2.54,P<0.05),which was higher in summer simulation group than that of the winter simulation group.There were significant differences in NPY and AgRP between simulated groups in summer and winter(NPY:F=-2.98,P<0.05;AgRP:F=-2.05,P<0.05).NPY and AgRP were higher in winter simulation group than those in summer simulation group.All of the above results suggested that A.chevrieri adapted to cold conditions similar to winter mainly by reducing body mass,increasing food intake,metabolic rate and the expression of orexigenic neuropeptides gene in hypothalamus.

关 键 词：高山姬鼠 体重 产热特征 季节模拟 

分 类 号：Q955[生物学—动物学] Q958