玉米HSP90基因家族的全基因组鉴定与分析  

Genome-Wide Identification and Analysis of HSP90 Gene Family in Maize

作　　者：曹丽茹[1] 王国瑞 张新[1] 魏良明[1] 魏昕[1] 张前进[1] 邓亚洲 王振华[1] 鲁晓民[1] Cao Liru;Wang Guorui;Zhang Xin;Wei Liangming;Wei Xin;Zhang Qianjin;Deng Yazhou;Wang Zhenhua;Lu Xiaomin(Grain Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院粮食作物研究所,河南郑州450002

出　　处：《作物杂志》2021年第5期28-34,共7页Crops

基　　金：河南省农业科学院杰出青年发展基金(2020JQ02)。

摘　　要：热激蛋白90(HSP90)广泛参与生物体的生长发育,且积极响应多种非生物胁迫。为全面解析玉米HSP90基因家族信息,对HSP90基因家族进化树、基因结构、保守基序(motif)、GO富集和组织表达模式等进行分析,并进一步分析ZmHSP90-1基因响应干旱胁迫的表达模式及蛋白互作关系。结果显示,从玉米全基因组水平共鉴定到11个ZmHSP90家族基因,被划分为5个亚族(I~V);相邻进化树分支上的ZmHSP90基因具有相似的motif和基因结构;GO富集分析显示,11个ZmHSP90基因均参与了蛋白质折叠过程;表达模式分析显示,11个ZmHSP90基因在不同组织或器官中均有表达,其中ZmHSP90-1基因的表达量相对较高;ZmHSP90-1基因在耐旱性强的玉米自交系中具有更高的表达量,且积极响应干旱和复水过程;蛋白互作预测显示,ZmHSP90-1可能与其互作蛋白协同响应非生物胁迫,发挥生物学功能。Heat shock protein 90(HSP90) are widely involved in the growth and development of organisms and actively responds to a variety of abiotic stresses. In order to analyze HSP90 gene family of maize, the phylogenetic tree gene structure, conserved motif, GO enrichment and tissue expression pattern of the family gene were analyzed, and further analyzed the expression pattern in response to drought stress and protein interaction relationship. The results showed that the eleven Zm HSP90 genes were identified from the whole genome level of maize and ZmHSP90 were divided into five groups(I-V). Zm HSP90 genes on adjacent phylogenetic tree branches had similar gene structure and motif. GO enrichment analysis showed that the eleven Zm HSP90 genes were involved in protein folding process. The expression pattern analysis showed that the eleven ZmHSP90 genes were expressed in different tissues or organs, and Zm HSP90-1 had relatively higher expression. Zm HSP90-1 gene had a higher expression level in drought-tolerant maize inbred lines, and responds to drought and rewater processes rapidly. Protein interaction prediction showed that Zm HSP90-1 might cooperate with its interacting protein to respond to abiotic stress, thus exerting its biological function.

关 键 词：玉米 热激蛋白90 表达水平 干旱响应 蛋白互作 

分 类 号：S513[农业科学—作物学]