白果蒲桃组织培养技术  

Tissue culture technique of Syzygium album

作　　者：陈春[1] CHEN Chun(Fujian Forestry Science and Technology Test Center,Nanjing,Fujian 363600,China)

机构地区：[1]福建省林业科技试验中心,福建南靖363600

出　　处：《亚热带农业研究》2021年第3期200-204,共5页Subtropical Agriculture Research

基　　金：漳州市自然科学基金项目(ZZ2021J22)。

摘　　要：[目的]优化白果蒲桃种子无菌播种及组织培养的最佳条件。[方法]应用正交设计及多重比较等方法,开展白果蒲桃无菌体系建立、种子萌发诱导、不定芽增殖及生根培养等研究。[结果]以白果蒲桃种子为外植体,0.1%HgCl 2的最佳消毒时间为30 min,最佳萌发培养基为:MS+0.2 g·L^(-1)活性炭+0.4 g·L^(-1)蛋白胨+30 g·L^(-1)蔗糖+5.0 g·L^(-1)琼脂,萌发天数为32.4 d,萌发率达94.5%,苗生长健壮;最佳增殖培养基为:1/2MS+0.8 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1)蔗糖+5.0 g·L^(-1)琼脂;不定芽最佳生根诱导培养基为:1/2MS+0.4 mg·L^(-1) IBA+0.2 mg·L^(-1) NAA+0.2 g·L^(-1)活性炭+15 g·L^(-1)蔗糖+7.0 g·L^(-1)卡拉胶,平均根数为3.5根,平均生根率达70.9%。[结论]以白果蒲桃种子为外植体,可建立白果蒲桃组织培养技术体系,为其优良品种的选育提供参考。[Purpose]To optimize the cultivation condition for aseptic seeding and tissue culture of Syzygium album.[Method]The establishment of aseptic system,induction of seed germination,adventitious bud proliferation and rooting culture were studied by orthogonal design and random block design.[Result]The optimal procedure for aseptic culture system was to disinfect seeds in 0.1%HgCl 2 for 30 min,and to use the germination medium containing MS+0.2 g·L^(-1) activated carbon+0.4 g·L^(-1) peptone+30 g·L^(-1) sucrose+5.0 g·L^(-1) agar.The germinating period lasted for 32.4 d,resulting in a germination rate was 94.5%.The optimal proliferation medium for adventitious buds was 1/2MS+0.8 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1) sucrose+5.0 g·L^(-1) agar,and the best rooting medium for adventitious buds consisted of 1/2MS+0.4 mg·L^(-1) IBA+0.2 mg·L^(-1) NAA+0.2 g·L^(-1) activated carbon+15 g·L^(-1) sucrose+7.0 g·L^(-1) carrageenan,yielding 3.5 roots and 70.9%rooting rate at average.[Conclusion]S.album seed can be used as the explant to establish an effective tissue culture system,to provide high-quality seedlings for landscaping and technical support for the breeding of excellent varieties.

关 键 词：白果蒲桃 无菌播种 组织培养 

分 类 号：S723.132.1[农业科学—林木遗传育种]