基于种特异引物(SS-COI)快速鉴定锈红果实蝇  

Rapid Identification of Bactrocera rubigina by Species-specific Primers(SS-COI)

作　　者：黄振[1,2] 郭琼霞 侯有明 HUANG Zhen;GUO Qiong-xia;HOU You-ming(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;Fuzhou Changle Airport Customs,Fuzhou,Fujian 350209;Fuzhou Customs Technology Center,Fuzhou,Fujian 350001)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]福州长乐机场海关,福建福州350209 [3]福州海关技术中心,福建福州350001

出　　处：《安徽农业科学》2021年第21期164-166,206,共4页Journal of Anhui Agricultural Sciences

基　　金：福建省自然科学基金项目(2011J01066,2012JO1061)。

摘　　要：[目的]开展不受虫态限制的实蝇快速鉴定技术研究。[方法]基于mtDNA COⅠ基因序列,筛选设计了一对能够准确鉴定锈红果实蝇的种特异性引物XF29和XR293,选用锈红果实蝇作为阳性对照,颜带实蝇B.cilifer(Hendel)、瘤胫实蝇B.tuberculata(Bezzi)等19种实蝇作为阴性对照,进行PCR扩增和电泳检测。[结果]仅阳性种锈红果实蝇在约265 bp的位置扩增出一条清晰且单一的条带,其余实蝇均未出现任何条带。[结论]采用SS-PCR技术,基于mtDNA COⅠ基因序列筛选设计的特异引物种特异性和稳定性强,可为进出境果蔬检疫、疫情早期预警和有效监测防治提供快速鉴定的依据。[Objective]To study the rapid identification technology of Bactrocera rubigina.[Method]Based on mtDNA COⅠgene sequence,a pair of species-specific primers XF29 and XR293 were screened and designed,which could accurately identify Bactrocera rubigina.Bactrocera rubigina was selected as positive control,and 19 fruit flies such as B.cilifer and B.tuberculata were selected as negative control for PCR amplification and electrophoresis detection.[Result]Only the positive fruit fly rust red fruit fly amplified a clear and single band at about 265 bp,and none of the other fruit flies showed any band.[Conclusion]Using SS-PCR technology,the specific primers designed based on mtDNA COⅠgene sequence screening have strong species specificity and stability,which can provide rapid identification basis for entry and exit fruit and vegetable quarantine,early warning of epidemic situation and effective monitoring and control.

关 键 词：锈红果实蝇 SS-PCR技术 COⅠ基因 种特异性引物 分子鉴定 

分 类 号：S433[农业科学—农业昆虫与害虫防治]