小麦水通道蛋白基因家族的全基因组鉴定及其在盐和干旱胁迫响应中的作用  

Genome-wide identification of the aquaporin gene family in wheat and their roles in salt and drought stress response

作　　者：马东方[1,2] 胡义锋 牛红莉 曾凡松[2] MA Dongfang;HU Yifeng;NIU Hongli;ZENG Fansong(Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou 434025,Hubei;Key Laboratory of Integrated Pest Management on Crop in Central China,Ministry of Agriculture,Hubei Province Key Laboratory for Control of Crop Diseases,Pest and Weeds,Institute of Plant Protection and Soil Science,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei)

机构地区：[1]湿地生态与农业利用教育部工程中心,主要粮食作物产业化湖北省协同创新中心(长江大学),湖北荆州434025 [2]农业部华中作物有害生物综合治理重点实验室,农作物重大病虫草害防控湖北省重点实验室,湖北省农业科学院植保土肥研究所,湖北武汉430064

出　　处：《长江大学学报（自然科学版）》2021年第5期84-103,共20页Journal of Yangtze University(Natural Science Edition)

基　　金：National Key R&D Program of China“Integrated study and demonstration of chemical fertilizer and pesticide reduction techniques for winter wheat in the Yangtze River Basin”(2018YFD0200506);National Natural Science Foundation of China“Fine Mapping of Stripe Rust Resistance Gene YrBai in adult plant of wheat cultivar Baidatou”(31501620).

摘　　要：水通道蛋白(AQPs)的功能是选择性地控制水和其他小分子通过细胞膜的流动,它们在植物的许多生理过程中都是至关重要的,包括非生物胁迫反应。小麦(Triticum aestivum L.)是全球重要的粮食作物,但干旱和盐胁迫是小麦生长和产量形成的重要影响因素。共鉴定得到了127个非冗余的小麦水通道蛋白基因和4个可变剪接体。对TaAQP基因进行了RNA-seq分析,揭示了小麦AQP基因的特异性表达模式。其中,TaTIPs和TaPIPs的表达高于TaNIPs和TaSIPs。qRT-PCR分析表明,小麦在干旱和盐胁迫条件下,TaNIP4;03_3D,TaTIP2;02b_7B,TaSIP2;02_4A,TaNIP3;03_6D和TaNIP2;04a_7D等AQPs受到显著诱导并且有高表达量,表明它们参与了胁迫响应。这些结果为进一步探索TaAQPs基因在植物应对干旱胁迫和盐胁迫中的作用提供了新的思路。Aquaporins(AQPs)function in selectively controlling the flow of water and other small molecules through cell membranes.They are essential for many physiological processes in plants,including the abiotic stress response.Wheat(Triticum aestivum L.)is one of the most important food crops globally,but the effects of drought and salt stress on the growth and yield are significant constraints.127 non-redundant wheat aquaporin genes(s)and 4 alternative splicing variants were identified.RNA-seq analysis of TaAQP genes reveals specific expression patterns of wheat AQP genes.Specifically,TaTIPs and TaPIPs are more highly expressed than TaNIPs and TaSIPs.qRT-PCR analysis shows that when wheat is subjected to drought and salt stress,several AQPs,including TaNIP4;03_3D,TaTIP2;02b_7B,TaSIP2;02_4A,TaNIP3;03_6D and TaNIP2;04a_7D,are significantlyinduced and have high expression,suggesting that they participate in stress response.These results provide new ideas for further exploring the role of TaAQPs genes in plant response to drought stress and salt stress.

关 键 词：非生物胁迫 AQP 干旱胁迫 基因表达 盐胁迫 普通六倍体小麦 

分 类 号：Q78[生物学—分子生物学] Q945.78