一测多评法同时测定蒙药述达格-4中5个黄酮类成分的含量  

Simultaneous determination of five flavonoids in Mongolian medicine Shudage-4 by QAMS

作　　者：嵇增云 贾鑫[2,3] 赵海龙 董玉 JI Zeng-yun;JIA Xin;ZHAO Hai-long;DONG Yu(College of Pharmacy,Inner Mongolian Medical University,Hohhot 010110,China;Department of Pharmacy,Inner Mongolia International Mongolian Hospital,Hohhot 010059,China;Inner Mongolia Engineering Research Center for Effective Substances and Quality Control of Traditional Mongolian Medicine,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110 [2]内蒙古医科大学附属医院药剂部,呼和浩特010059 [3]内蒙古自治区蒙药药效物质与质量控制工程技术研究中心,呼和浩特010110

出　　处：《药物分析杂志》2021年第9期1605-1611,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(82060773);内蒙古自治区自然科学基金项目(2019ZD16)。

摘　　要：目的:建立一测多评法同时测定蒙药述达格-4总黄酮提取物中槲皮素、山柰酚、高良姜素、山柰素、高良姜素-3-甲醚5个黄酮类成分的含量。方法:采用Agilent C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇(B)-0.5%乙酸水溶液(A),梯度洗脱,流速0.6 mL·min^(-1),柱温30℃,进样量20μL,检测波长:286 nm检测山柰酚、山柰素、高良姜素-3-甲醚,256 nm检测槲皮素。以高良姜素为内参,计算其他4个黄酮类成分的相对校正因子,采用该相对校正因子计算其他4个成分的含量,实现一测多评。同时采用外标法测定5个黄酮类成分的含量,并对二者测定结果进行比较,验证一测多评法的可行性和准确性。结果:在一定线性范围内,以高良姜素为内参,槲皮素、山柰酚、山柰素、高良姜素-3-甲醚4个黄酮类成分的平均校正因子分别为0.0948、0.2266、0.2670、0.1554,且一测多评法测定结果与外标法测定结果无显著差异。结论:建立了同时测定蒙药述达格-4总黄酮提取物中5个黄酮类成分含量的一测多评法,该法快速、准确、简便、可靠,可用于蒙药述达格-4的质量控制研究。Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)method for simultaneous determination of five flavonoids(quercetin,kaempferol,galangin,kaempferide,and galangin-3-methylether)in total flavonoids extract of Mongolian medicine Shudage-4. Methods:HPLC analysis was performed on an Agilent C_(18) column(4.6 mm×250 mm,5 μm)with gradient elution of methanol(B)-0.5% acetic acid in water(A)at a flow rate of 0.6 mL·min^(-1). The column temperature was 30 ℃ and the injection volume was 20 μL. The detection wavelength was set at 254 nm for quercetin and kaempferol,286 nm for kaempferol,galangin,kaempferide and galangin-3-methylether. Galangin was selected as the internal reference substance,the relative correction factors(RCFs)between galangin and the other fourflavonoids were established,and the contents of the five constituents in samples were calculated to realize QAMS.At the same time,the external standard method was used to quantify contents of the five constituents,and the two methods were compared to validate the accuracy and feasibility of the new method. Results:Within a certain linear range,the RCFs of quercetin,kaempferol,kaempferide and galangin-3-methylether were 0.094 8,0.226 6,0.267 0,and 0.155 4 respectively. With galangin as the internal reference,there was no significant difference between the quantitative results of QAMS and external standard method(ESM). Conclusion:The AQAMS method for the simultaneous determination of five flavones in total flavonoids extract of Mongolian medicine Shudage-4 was established. This method is rapid,accurate,simple and reliable,and can be used for the quality control study of Mongolian medicine Shudage-4.

关 键 词：蒙药述达格-4 一测多评法 相对校正因子 黄酮类化合物 高良姜素 

分 类 号：R917[医药卫生—药物分析学]