临床微生物检验中细菌耐药性分析  

Analysis of bacterial drug resistance in clinical microbiological examination

作　　者：李平[1] LI Ping(Department of Laboratory Medicine,Pizhou Hospital of Traditional Chinese Medicine in Jiangsu Province,Jiangsu,Pizhou 221300,China)

机构地区：[1]江苏省邳州市中医院检验科,江苏邳州221300

出　　处：《中国医药科学》2021年第19期149-151,共3页China Medicine And Pharmacy

摘　　要：目的探讨在临床微生物检验中的细菌耐药性。方法选取2019年3月至2020年12月邳州市中医院检验科收集的580份微生物标本,对细菌耐药性进行监测。采用全自动微生物鉴定/药敏分析(VITEK)系统对细菌自动化鉴定试验,用Excel统计检测结果,并进行数据分析。结果580份标本耐药菌株中,凝固酶阴性葡萄球菌耐药性占比最高(5.34%),其次是金黄色葡萄球菌(3.45%);革兰阴性耐药菌中,铜绿假单胞菌对青霉素、氨苄西林、头孢他啶、左氧氟沙星、克林霉素、阿奇霉素耐药性均较高;革兰阳性耐药菌中,凝固酶阴性葡萄球菌对氨苄西林、头孢他啶、左氧氟沙星、克林霉素、阿奇霉素耐药性较高。凝固酶阴性葡萄球菌、金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌主要分布于ICU,大肠埃希菌、肺炎克雷伯杆菌主要分布于呼吸内科。结论细菌耐药性监测在临床微生物检验中的应用价值较高,有利于临床医师依据细菌耐药性检测结果选取最为适宜的治疗方案,促使患者早日康复。Objective To investigate the bacterial drug resistance in clinical microbiological examination.Methods A total of 580 microbiology specimens collected from the laboratory of Pizhou Hospital of Traditional Chinese Medicine from March 2019 to December 2020 were selected to monitor bacterial drug resistance.The automated microbiological identification/susceptibility analysis(VITEK)system was used to perform automated bacterial identification test.Excel was utilized to count the test results and data analysis was conducted.Results Among the 580 specimens of drug-resistant strains,coagulase-negative staphylococci accounted for the highest proportion(5.34%),followed by staphylococcus aureus(3.45%).Among gram-negative resistant bacteria,pseudomonas aeruginosa showed high-level resistance to penicillin,ampicillin,ceftazidime,levofloxacin,clindamycin,and azithromycin;Among gram-positive resistant bacteria,coagulase-negative staphylococci showed high-level resistance to ampicillin,ceftazidime,levofloxacin,and clindamycin,azithromycin.Coagulase-negative staphylococci,staphylococcus aureus,pseudomonas aeruginosa,and acinetobacter baumannii were mainly distributed in ICU.Escherichia coli and klebsiella pneumoniae were mainly distributed in respiratory medicine.Conclusion Bacterial drug resistance monitoring has a high application value in clinical microbiological examination.It is helpful for clinicians to select the most appropriate treatment plan based on the results of bacterial drug resistance testing,and promote patient's early recovery.

关 键 词：细菌 耐药性监测 临床微生物 革兰阴性菌 革兰阳性菌 

分 类 号：R446.5[医药卫生—诊断学]