聚乳酸定向大孔细胞支架的制备与表征  

Preparation and Characterization of Poly(D,L-Lactic Acid)Directional Macroporous Cell Scaffolds

作　　者：王友法[1] 王雅楠 WANG Youfa;WANG Yanan(State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,Hubei,China;School of Materials Science and Engineering,Wuhan University of Technology,Wuhan 430070,Hubei,China)

机构地区：[1]武汉理工大学材料复合新技术国家重点实验室,湖北武汉430070 [2]武汉理工大学材料科学与工程学院,湖北武汉430070

出　　处：《武汉大学学报（理学版）》2021年第5期496-502,共7页Journal of Wuhan University:Natural Science Edition

基　　金：国家级大学生创新创业训练计划(S202010497023);国家自然科学基金(51172172);武汉市科技攻关项目(201160923310)。

摘　　要：采用改进的热致相分离和冷冻干燥相结合的技术,制备了一系列不同孔结构的聚乳酸定向大孔细胞支架材料,用扫描电子显微镜进行表征,探讨了聚乳酸初始浓度、温度梯度和冷却制度对细胞支架形貌及孔结构的影响。结果表明,聚乳酸初始浓度和温度梯度决定了细胞支架的孔道分布、孔隙率和孔径大小,冷却制度决定了支架内部结构的取向形态;使用改进方法,在0℃低温介质制备的5%聚乳酸定向大孔细胞支架形貌和结构最优,孔隙率高(90.3%)、孔径大(342±28μm)、孔壁厚度(10μm)合适,具有较好的力学强度和生物可降解性,且生物相容性良好。A series of poly(D,L-lactic acid)(PDLLA)directional macroporous cell scaffolds were prepared via improved thermal induced phase separation(TIPS)and freeze-drying technique and characterized by scanning electron microscope(SEM).The effects of PDLLA initial concentrations,temperature gradients and lyophilization process on scaffolds morphology and pore structure were investigated.The results show that the PDLLA initial concentrations and the gradients of temperature determine the pore channel distribution,porosity and pore diameter of the cell scaffolds,while the lyophilization method determines the orientation morphology.5%PDLLA directional macroporous scaffolds prepared by the improved method under 0℃have the best morphology,with higher porosity(90.3%),bigger pore size(342±28μm)and moderate wall thichness(10μm).And the biological properties results show that the PDLLA scaffolds can be degraded in phosphate buffer solution(PBS)and have good biocompatibility.

关 键 词：聚乳酸 热致相分离 支架材料 定向孔结构 

分 类 号：R318.08[医药卫生—生物医学工程]