高效液相色谱法同时测定葛根中4种异黄酮类成分  

Determination of four kinds of Isoflavonoids in Puerariae Radix

作　　者：王晓莉[1] 李思齐 宋爱华[2] 韩飞[2] Wang Xiaoli;Li Siqi;Song Aihua;Han Fei(Jiangsu Hengrui Medicine Co.,Ltd.,Lianyungang 222047,China;Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]江苏恒瑞医药股份有限公司研究院,江苏连云港222047 [2]沈阳药科大学,辽宁沈阳110000

出　　处：《云南化工》2021年第9期61-63,共3页Yunnan Chemical Technology

摘　　要：采用高效液相色谱法对10批次葛根药材中4种异黄酮类成分同时进行含量测定。以Agilent Eclipse XDB-C_(18)色谱柱(250 mm×Φ4.6 mm,5μm)为固定相,以乙腈-0.1%甲酸水(体积分数)溶液为流动相,梯度洗脱,流速0.8 m L/min,检测波长254 nm,柱温30℃。结果表明,3’-羟基葛根素、葛根素、3’-甲氧基葛根素、葛根素芹菜糖苷在一定浓度范围内与峰面积线性关系良好(r≥0.9998,n=6),精密度、重复性和稳定性的峰面积RSD值小于2.0%,加样回收率在97.6%~103.6%。所建立的含量测定方法简便快捷,准确可靠,为葛根药材化学成分定量研究提供了参考。An HPLC method was applied to simultaneously quantify four kinds of chemical components in Puerariae Radix.An Agilent Eclipse XDB-C_(18) column(250 mm × Φ4.6 mm,5 μm) was adopted.The mobile phase consisted of acetonitrile and 0.1% formic acid-water(v/v) was used for gradient elution.The flow rate was 0.8 m L/min,detection wavelength was 254 nm and column temperature was 30 ℃.The methodology results showed that the content of 3’-hydroxypuerarin,mirificin,puerarin and 3’-methoxypuerarin exhibited good linearity with chromatographic peak area in a certain rang(r≥0.9998,n = 6).The RSDs of precision,stability and repeatability were lower than 2.0%,the recoveries were 97.6% ~ 103.6%,which were all in accord with the standards of Chinese Pharmacopoeia.The established HPLC method is simple,accurate and reliable,and it provides reference for PR quantitative research.

关 键 词：高效液相色谱法 葛根 异黄酮 含量测定 

分 类 号：R284[医药卫生—中药学]