枫泾猪、梅山猪和沙乌头猪全基因组ROH检测及候选基因鉴定  

Genome-wide scan for run of homozygosity and identification of corresponding candidate genes in Fengjing pigs,Meishan pigs and Shawutou pigs

作　　者：赵旭庭[1] 徐盼[1] 马政 仲德 刘林雨 倪黎纲[1] 吴井生[2] ZHAO Xu-ting;XU Pan;MA Zheng;ZHONG De;LIU Lin-yu;NI Li-gang;WU Jing-sheng(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Jiangsu Vocational College of Agriculture and Forestry,Jurong 212400,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]江苏农林职业技术学院,江苏句容212400

出　　处：《江苏农业学报》2021年第5期1234-1243,共10页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金(31702089);江苏现代农业(生猪)产业技术体系[JATS(2020)346];江苏高校“青蓝工程”项目。

摘　　要：通过检测枫泾猪、梅山猪和沙乌头猪的长纯合片段(Runs of homozygosity,ROH),鉴定其与3个猪种经济性状相关的候选基因。利用全基因组单核苷酸多态性(Single Nucleotide Polymorphisms,SNP)芯片数据对这3个猪群体进行全基因组ROH检测,统计ROH数量、长度及分布,计算基于ROH的近交系数(FROH),并在高频ROH区域鉴定候选功能基因。结果显示,从枫泾猪、梅山猪和沙乌头猪群体中分别检测到1588个、3314个和4419个ROH。枫泾猪的ROH平均长度为12.84 Mb,平均FROH为0.084;梅山猪的ROH平均长度为10.55 Mb,平均FROH为0.164;沙乌头猪的ROH平均长度为10.52 Mb,平均FROH为0.141。在高频ROH区域共鉴定到9个枫泾猪候选功能基因和8个梅山猪候选功能基因。研究结果为枫泾猪、梅山猪和沙乌头猪的资源保护和分子标记辅助选择奠定了基础。By detecting runs of homozygosity(ROH)of Fengjing pig,Meishan pig and Shawutou pig,the candidate genes related to the economic traits of the three pig breeds were identified.The genome-wide single nucleotide polymorphisms(SNP)chip data were used to perform genome-wide ROH scan for the three pig breeds.The number,length and distribution of ROH were counted,and the inbreeding coefficient(FROH)was calculated based on ROH.In addition,the functional candidate genes were identified in the high frequency ROH region.The results showed that 1588,3314 and 4419 ROH were detected in the three pig populations,respectively.The average length of ROH was 12.84 Mb and the average FROH was 0.084 in Fengjing pigs.The average length of ROH was 10.55 Mb and the average FROH was 0.164 in Meishan pigs.The average length of ROH was 10.52 Mb and the average FROH was 0.141 in Shawutou pigs.Nine functional candidate genes of Fengjing pig and eight functional candidate genes of Meishan pig were identified in high frequency ROH region.The results lay a foundation for resource conservation and molecular marker-assisted selection of Fengjing pig,Meishan pig and Shawutou pig.

关 键 词：猪 长纯合片段 近交系数 候选基因 

分 类 号：S813.1[农业科学—畜牧学]