基于分子识别的免疫层析技术用于新冠肺炎感染的快速诊断  

Lateral Flow Assay Based on Molecular Recognition for Diagnosis of Corona Virus Disease 2019 Infection

作　　者：刘园 邓瑾琦 赵帅[2] 田飞 李轶[1] 孙佳姝 刘超[2,3] LIU Yuan;DENG Jinqi;ZHAO Shuai;TIAN Fei;LI Yi;SUN Jiashu;LIU Chao(Tianjin Key Laboratory of Molecular Optoelectronic Sciences,Department of Chemistry,School of Science,Tianjin University&Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China;Beijing Engineering Research Center for BioNanotechnology,CAS Key Laboratory of Standardization and Measurement for Nanotechnology,CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China;School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]天津大学理学院化学系天津市分子光电子科学重点实验室,化学科学与工程协同创新中心,天津300072 [2]北京市纳米生物医学检测工程技术研究中心,中国科学院纳米标准与检测重点实验室,中国科学院纳米科学卓越创新中心,国家纳米科学中心,北京100190 [3]中国科学院大学未来技术学院,北京100049

出　　处：《高等学校化学学报》2021年第11期3390-3405,共16页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22025402,91959101,21904028);中国科学院科研仪器设备研制项目(批准号:YJKYYQ20190068);中国科学院基础前沿科学研究计划从0-1原始创新项目(批准号:ZDBS-LY-SLH025);中国科学院战略性先导科技专项(批准号:XDB36000000);国家重点研发计划项目(批准号:2020YFA0210804);北京市青年拔尖个人项目(批准号:2018000021223ZK44)资助。

摘　　要：目前由新型冠状病毒(SARS-CoV-2)引发的新冠肺炎疫情仍在全球蔓延.快速筛查并隔离感染者(包括无症状感染者)是遏制疫情传播的重要手段之一.免疫层析技术是一种相对成熟的快速检测技术,由于其操作简单、反应时间短且结果稳定,在生物标志物检测领域具有广阔的应用前景.本文总结了目前免疫层析检测技术在新冠肺炎感染筛查领域的研究进展,涵盖病毒抗体、蛋白、核酸等检测靶标,并对不同检测方法的优势、局限性进行了简要评述,最后简单介绍了目前用于新冠肺炎感染筛查的免疫层析试纸的实际应用情况.The novel coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still spreading worldwide.Rapid screening and isolation of infected individuals,including asymptomatic individuals,is one of the key steps to control the epidemic.Lateral flow assay is a mature technique for point-of-care detection.Owing to its advantages of simple operation,fast response and robustness,lateral flow assay has been a powerful tool for rapid detection of biomarkers.This paper summarized the recent research progresses of lateral flow assays for the diagnosis of CO VID-19 infection,with antibody,protein,or nucleic acid as detection targets,and the advantages and limitations of different detection methodologies were shortly discussed.Finally,we briefly introduced the currently commercialized lateral flow test strips for COVID-19 diagnostics.

关 键 词：分子识别 免疫层析检测 新冠肺炎 新型冠状病毒 即时检测 

分 类 号：O652[理学—分析化学]