多指标免疫分析的研究进展  

Research Progress of Multiplex Immunoassay

作　　者：罗成 彭雅梅 沈宏[1] 方群[1,2] 潘建章 LUO Cheng;PENG Yamei;SHEN Hong;FANG Qun;PAN Jianzhang(Department of Chemistry,Zhejiang University,Hangzhou 310058,China;Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou 311200,China)

机构地区：[1]浙江大学化学系,杭州310058 [2]浙江大学杭州国际科创中心,杭州311200

出　　处：《高等学校化学学报》2021年第11期3421-3432,共12页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21974122,21827806);浙江省自然科学基金(批准号:LY19B050002)资助。

摘　　要：免疫分析是临床上检测生理相关蛋白质指标的主要方法.与单一指标的免疫分析相比,临床体外诊断对多指标的生理相关蛋白质免疫分析有着更为广泛的需求.通过在固相载体上完成免疫反应的非均相免疫分析具有灵敏度高的优点,是当前多指标免疫分析的主流方法.本文按照固相载体的不同,对近年来的多指标免疫分析系统分别从技术原理、实现方法、各自特点等方面的研究进展进行综合阐述.最后对比总结了不同系统的优势与不足,并展望了微流控多指标免疫分析在即时检测领域的发展前景.Clinically,immunoassay is the main method to detect physiologically related protein indicators.Compared with the singleplex immunoassay,clinical in vitro diagnosis has a greater demand for the multiplex immunoassay of physiologically related proteins.The heterogeneous immunoassay method that complete the immune reaction on a solid-phase carrier has the advantage of high sensitivity,and has become the main method of multiplex immunoassay at present.According to the different solid-phase,the research progress of multiplex immunoassay systems in recent years has been reviewed in terms of technical principles,methods,and characteristics.Finally,the advantages and disadvantages of different systems are compared and summarized,and the development of microfluidic multiplex immunoassay in the point of caring testing(POCT)field is prospected.

关 键 词：免疫分析 多指标 微流控 即时检测 

分 类 号：O657[理学—分析化学]