二氧化硅纳米颗粒表面原位还原银纳米簇电化学发光传感界面的构建与分子识别  

Construction of Electrochemiluminescence Sensing Interface Based on Silver Nanoclusters-Silica Nanoparticles and Biomolecular Recognition

作　　者：王博东 潘美辰 卓颖[1] WANG Bodong;PAN Meichen;ZHUO Ying(Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)

机构地区：[1]发光分析与分子传感教育部重点实验室(西南大学),西南大学化学化工学院,重庆400715

出　　处：《高等学校化学学报》2021年第11期3519-3525,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22022408);重庆市英才计划青年拔尖人才项目(批准号:CQYC201905067)资助。

摘　　要：通过牛血清蛋白(BSA)对二氧化硅纳米颗粒(SiO_2 NPs)表面进行氨基、巯基功能化,随后以BSA同时作为模板和还原剂,原位生成银纳米簇(Ag NCs),获得显著增强阴极电化学发光(ECL)信号的Ag NCs-SiO_(2) NPs复合纳米材料.结果表明,当测试溶液中含有L-半胱氨酸(L-Cys)时,其与传感界面上的Ag NCs发生共价结合作用,从而猝灭其ECL信号.基于该原理,构建了"开-关"型ECL信号响应模式的L-Cys生物传感器.该传感器检测L-Cys的浓度范围为50 nmol/L~50μmol/L,最低检测限达到13.7 nmol/L,能够实现L-Cys的高灵敏及特异性分析,有望在生物、医学等领域得到广泛应用.A highly sensitive L-Cys(L-cysteine)biosensor was constructed via silver nanoclusters loaded on the surface of silica nanoparticles(Ag NCs-SiO_(2) NPs)as an electrochemiluminescence(ECL)nanocomposite modified on the surface of glassy carbon electrode.The surface of silica nanoparticles(SiO_(2) NPs)was functionalized with amino and sulfhydryl groups by bovine serum protein(BSA),and then silver nanoclusters(Ag NCs)were in situ reduced on the surface of SiO_(2) NPs by using BSA as a template and a reducing agent,which realized the stable load of silver nanoclusters.The Ag NCs-SiO2 NPs electrochemiluminescence nanocomposites with the enhanced cathodic ECL signal were successfully obtained.With the existence of L-Cys in the solution,it covalently binds to Ag NCs forming Ag-S bond and quenching the ECL signal.Based on the above principle,a biosensor with the on-off signal response mode is constructed for the detection of L-Cys.The concentration range of L-Cys detected by the biosensor is 50 nmol/L-50μmol/L.At the same time,the detection limit is 13.7 nmol/L,which can realize the sensitivity and specificity analysis of L-Cys.The biosensor constructed above is expected to be extensively applied in biological,medical and other fields.

关 键 词：电化学发光 银纳米簇 二氧化硅纳米颗粒 生物传感器 L-半胱氨酸 

分 类 号：O657[理学—分析化学]