2型糖尿病差异表达基因筛选及其调控网络构建  

On Screening Differentially Expressed Genes of Type 2 Diabetes Mellitus and the Construction of T2DM Control Network

作　　者：程学英 彭益彬 孙杰 潘凌鸿[2,3] 刘奔 CHENG Xueying;PENG Yibin;SUN Jie;PAN Linghong;LIU Ben(Department of Neurology,The Affiliated Hospital of Putian University,Putian Fujian 351100,China;Pharmaceutical and Medical Technology College,Putian University,Putian Fujian 351100,China;Key Laboratory of Medical Microecology(Putian University),Fujian Province University,Putian Fujian 351100,China)

机构地区：[1]莆田学院附属医院神经内科,福建莆田351100 [2]莆田学院药学与医学技术学院,福建莆田351100 [3]莆田学院医学微生态学福建省高校重点实验室,福建莆田351100

出　　处：《莆田学院学报》2021年第5期41-48,共8页Journal of putian University

基　　金：福建省自然科学基金资助项目(2020J01903);莆田市科技计划项目(2019S3F007);莆田学院科学研究项目(2019013)。

摘　　要：运用生物信息学方法对2型糖尿病(T2DM)易感基因进行筛选、分析,从分子水平探讨T2DM发病机制。利用GEO数据库查找T2DM患者外周血单个核细胞差异表达的基因集;利用DAVID数据库进行GO功能富集分析、KEGG信号通路分析;利用STRING工具与Cytoscape软件及MCODE插件对差异表达基因做蛋白质互作网络分析。结果如下:1)GO功能富集分析显示T2DM差异表达基因主要分布在G蛋白偶联受体信号通路、细胞增殖分化、信号转导等生物过程中,大部分与神经传导相关;2)KEGG信号分析结果显示差异表达基因主要集中在神经活性配体-受体相互作用信号通路、Ras信号通路等通路;3)共得到可信度较高的RAC1、PIK3R1、CDC42等12个关键基因。可见T2DM的发病受到炎症反应、氧化应激及神经信号转导等多种因素的影响。Based on the screening and analysis of the susceptibility genes of Type 2 Diabetes Mellitus(T2DM),this paper explores the pathogenesis of T2DM by using bioinformatics analysis method.Within the GEO database,we find the gene set of differential expression of peripheral blood mononuclear cells in type 2 diabetic patients.DAVID database is used for the analysis of GO function enrichment and KEGG signal pathway.String,Cytoscape and MCODE plug-in are used to analyze protein interaction network of differentially expressed genes.The results are as follows:1)GO functional enrichment analysis shows that those differentially expressed genes play a major role in biological processes such as G protein-coupled receptor signaling pathway,cell proliferation and differentiation,signal transduction,most of which are related to neuritis conduction;2)The results of KEGG signal analysis show that differentially expressed genes are mainly distributed in the neuroactive ligand-receptor interaction signaling pathway and Ras signaling pathway;3)12 key genes including RAC1,PIK3R1,CDC42,etc.are reliably obtained.Hence,we come to the conclusion that the pathogenesis of T2DM involves many factors,such as inflammatory response,oxidative stress,hormone stimulation response change,and neural signal transduction,etc.

关 键 词：2型糖尿病 基因 差异表达 调控 生物信息学 

分 类 号：R587.1[医药卫生—内分泌]