基于层积的文冠果种子破除休眠研究  

A Study on Seeds Dormancy Relief Based on Stratification on Xanthoceras Soebifolia Bunge

作　　者：刘春香[1] 陈忠秀 杨爱君 赵光强[1] LIU CHun-xiang;CHEN ZHong-xiu;YANG Ai-jun;ZHao Guang-qiang(Weifang University,Weifang,261061,China)

机构地区：[1]潍坊学院,山东潍坊261061

出　　处：《潍坊学院学报》2020年第6期1-6,14,共7页Journal of Weifang University

基　　金：潍坊市科技发展计划项目(2017ZJ1123);山东省自然科学基金(ZR2014JL017)。

摘　　要：文冠果是重要的木本油料作物,主要通过种子繁殖,种子自然条件下休眠率高,因此,提高文冠果的整齐出苗率对文冠果的种植有着十分重要的意义。本试验对文冠果种子进行6℃低温层积、-20℃低温、80℃高温、5%、10%浓度的H2O2和0.5molL-1NaOH碱液的处理,对休眠种子进行破除休眠试验,通过发芽势(用60d发芽率表示)和发芽率(用100d发芽率来表示)的比较,以及发芽结束后种子生活力的TTC染色试验,研究破除休眠的适宜条件。结果表明,6℃低温长期存放基本破除种子的休眠,但需要约3个月的处理时间,6℃低温层积结合5%的H2O2溶液1h浸泡处理显著提高了文冠果种子的发芽势,60d破除休眠率达97.1%,达到一般种子批发芽的基本要求。而6℃低温层积结合0.5mol·L-1NaOH溶液处理以及高温处理等都不同程度降低了文冠果种子的发芽势和发芽率。结论:可以通过6℃低温层积结合适宜浓度的H2O2破除文冠果种子休眠。Xanthoceras soebifolia Bunge is an importantly oil-bearing xylophyta, which is mainly propagated by seeds. The dormancy rate of seeds is high in natural conditions. Therefore, it is of great significance to improve the seedling emergence rate of Xanthoceras soebifolia Bunge for its cultivation. This experiment is to explore the proper condition of Xanthoceras soebifolia Bunge seeds by 6 ℃ cold,-20 ℃ sub-zero temperatures and 80 ℃ high temperatures, 5% and 10% H2O2 and 0.5 mol·L-1 NaOH, to break dormancy of dormant seeds, through the comparison of germination potential(represented by 60 d germination rate) and germination rate(represented by 100 d germination rate), followed by TTC staining experiment for seed viability after germination.The results show that 6℃ long time storage can break seed dormancy, but it needs more than 3 months. 6℃ cold stratification combined with 5% H2O2 solution soaking 1-hour treatment significantly increased germination potential of the seeds of Xanthoceras soebifolia Bunge, the dormancy broken rate was 97.1% at 60 d treatment, which meet general seeds germination rate requirements, However,the combination of 6 ℃ cold stratification with 0.5 mol·L-1 NaOH solution;or with and high temperature treatment reduced the germination potential and germination rate of the seeds. Conclusion: the dormancy of seeds can be broken by the combination of proper concentration of H2O2 with 6℃ cold stratification.

关 键 词：文冠果 破除休眠 低温 过氧化氢 

分 类 号：TN311[电子电信—物理电子学]