下丘脑POMC表达在miR-21敲除小鼠中的变化  

Changes of POMC expression in hypothalamus of miR-21 knockout mice

作　　者：朱艳娟 李晓晨 高忠爱 程静丽[3] 沈小芳 常宝成[2] Zhu Yanjuan;Li Xiaochen;Gao Zhongai;Cheng Jingli;Shen Xiaofang;Chang Baocheng(Department of Cardiology,Chu Hsien-I Memorial Hospital,Tianjin 300134,China;NHC Key Laboratory of Hormones and Development,Tianjin Key Laboratory of Metabolic Diseases,Chu Hsien-I Memorial Hospital&Tianjin Institute of Endocrinology,Tianjin Medical University,Tianjin 300134,China;Department of Nephrology,Chu Hsien-I Memorial Hospital,Tianjin 300134,China)

机构地区：[1]天津医科大学朱宪彝纪念医院心内科,300134 [2]天津医科大学朱宪彝纪念医院、天津市内分泌研究所、国家卫健委激素与发育重点实验室、天津市代谢性疾病重点实验室,300134 [3]天津医科大学朱宪彝纪念医院肾内科,300134

出　　处：《中华内分泌代谢杂志》2021年第10期943-947,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家重点研发计划(2018YFC1314000);国家自然科学基金(81774043、82074253);天津市科技计划项目(17ZXMFSY00140)。

摘　　要：目的研究阿黑皮素原(POMC)在miR-21敲除小鼠表达下丘脑中的变化及其相应的能量代谢变化。方法将miR-21敲除小鼠及野生型C57BL/6J小鼠分别分为糖尿病组和对照组,糖尿病组给予高脂饮食喂养联合小剂量链脲佐菌素腹腔注射,建立糖尿病模型。监测各组小鼠体重及血糖变化。实验结束后处死小鼠,取下丘脑组织检测POMC蛋白表达、STAT3 mRNA表达水平。结果基线时各组小鼠体重及血糖水平均无明显差异(P>0.05)。造模后分别于3、6、9、12周比较各组小鼠体重及血糖的差异,结果显示,糖尿病组及miR-21敲除糖尿病组小鼠体重在各时间点均高于对照组(P<0.05),并且,此2组间体重在3及12周时的差异也有统计学意义(P<0.05)。造模后糖尿病组小鼠血糖水平在各时间点均明显高于其他3组小鼠(P<0.05)。miR-21敲除糖尿病组小鼠在各时间点血糖水平均明显低于糖尿病组小鼠,而高于正常对照组,差异均具有统计学意义(P<0.05)。糖尿病小鼠下丘脑POMC蛋白及STAT3 mRNA水平较对照组明显降低,miR-21敲除的糖尿病小鼠下丘脑POMC蛋白及STAT3 mRNA水平较糖尿病组升高。结论miR-21敲除小鼠下丘脑POMC表达较未敲除miR-21糖尿病小鼠高,miR-21敲除可改善糖尿病小鼠血糖水平,减轻体重,改善能量代谢。Objective To investigate the changes of proopiomelanocortin(POMC)expression in hypothalamus and corresponding metabolism in miR-21 knockout mice.Methods miR-21 knockout or wild-type C57BL/6J mice were divided into diabetic group and control group,respectively.Diabetic mice model were forged with high-fat diet and low-dose streptozotocin.The changes of body weight and blood glucose in each group were monitored.By the end of the experiment,mice were sacrificed,and POMC protein expression and STAT3 mRNA expression in hypothalamus were detected.Results There were no significant differences in body weight and blood glucose levels among all groups at baseline(P>0.05).The differences of body weight and blood glucose levels among various groups were compared at 3,6,9 and 12 weeks after the model was established.The results showed that body weight of mice in the diabetes group or miR-21 knockout+diabetes group was higher than that in the control group at each time point(P<0.05).Moreover,there were significant difference in body weight between diabetes group and miR-21 knockout+diabetes group at 3 and 12 weeks(P<0.05).The blood glucose levels in diabetes group were significantly higher than those in other groups at each time point(P<0.05).The blood glucose level in miR-21 knockout+diabetes group was lower than that in diabetes group and higher than control group(P<0.05).POMC protein and STAT3 mRNA levels in diabetes group were significantly lower than those in control group,while those in the miR-21 knockout+diabetes group were higher than those in the diabetes group.Conclusions The expression of POMC in hypothalamus of miR-21 knockout mice is higher than that of wild-type diabetic mice.miR-21 knockout can decrease blood glucose level and body weight,and improve energy metabolism of diabetic mice.

关 键 词：微小RNA-21 能量代谢 糖尿病 2型 下丘脑 

分 类 号：R587.1[医药卫生—内分泌]