HPLC法同时测定五指柑中9种成分  

Simultaneous determination of nine constituents in Vitex negundo by HPLC

作　　者：陈丹[1,2] 戚建中 李柯 沈冰冰[1] 符国成 唐湘黔 龚小兵 CHEN Dan;QI Jian-zhong;LI Ke;SHEN Bing-bing;FU Guo-cheng;TANG Xiang-qian;GONG Xiao-bing(Hunan.Academy of Traditional Chinese Medicine,Changsha 410013,China;Xiangtan Medical and Health Vocational and Technical College,Xiangtan 411102 China;Huarun 39(Chenzhou)Pharmaceutical Co.,Ltd.,Chenzhou 423000,China)

机构地区：[1]湖南省中医药研究院,湖南长沙410013 [2]湘潭医卫职业技术学院,湖南湘潭411102 [3]华润三九(郴州)制药有限公司,湖南郴州423000

出　　处：《中成药》2021年第10期2733-2736,共4页Chinese Traditional Patent Medicine

基　　金：湖南省自然科学基金(2020JJ9005);郴州国家可持续发展议程创新示范区建设-湖南省科技重大专项(2019sfq13)。

摘　　要：目的建立HPLC法同时测定五指柑中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异荭草苷、荭草苷、异牡荆素、木犀草素-7-O-葡萄糖醛酸苷、木犀草素-3′-葡萄糖醛酸苷的含量。方法五指柑60%甲醇提取物的分析采用Welch ultimate C18色谱柱(4.6 mm×250 mm, 5μm);流动相乙腈-0.5%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长340 nm。结果 9种成分在各自范围内线性关系良好(r>0.999 5),平均加样回收率97.21%~98.39%, RSD 1.21%~1.87%。结论该方法准确稳定,重复性好,可用于五指柑的质量控制。AIM To establish an HPLC method for the simultaneous content determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoorientin, orientin, isovitexin, luteolin-7-O-glucuronide, luteolin-3′-glucuronide in Vitex negundo Linnaeus.METHODS The analysis of 60% methanol extract of V. negundo was performed on a 30 ℃ thermostatic Welch ultimate C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.5% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 340 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(r>0.999 5), whose average recoveries were 97.21%-98.39%, with the RSDs of 1.21%-1.87%.CONCLUSION This accurate, stable and reproducible method can be used for the quality control of V. negundo.

关 键 词：五指柑 化学成分 HPLC 

分 类 号：R284.1[医药卫生—中药学]