UPLC-MS法同时测定马兜铃果皮及种子中5种成分  

Simultaneous determination of five constituents in pericarps and seeds of Aristolochia herbs by UPLC-MS

作　　者：高昌琨[1] 高玉菊 张楠[2] 胡蝶[2] GAO Chang-kun;GAO Yu-ju;ZHANG Nan;HU Die(Department of Pharmacy,The First Affiliated Hospital of Anhui Medical University,The Grade 3 Chinese Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,Hefei 230022,China;Department of Pharmacy,The Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,China)

机构地区：[1]安徽医科大学第一附属医院药剂科国家中医药管理局中药化学三级实验室,安徽合肥230022 [2]安徽中医药大学第二附属医院药剂科,安徽合肥230061

出　　处：《中成药》2021年第10期2750-2754,共5页Chinese Traditional Patent Medicine

摘　　要：目的比较马兜铃药材果皮和种子中马兜铃酸A、B、C、D和马兜铃内酰胺的含量差异。方法UPLC-MS法同时测定马兜铃果皮及种子中5种马兜铃类成分的含量,分析采用Agilent Zorbax C_(18)色谱柱(2.1 mm×100 mm,1.8μm);流动相0.1%甲酸-乙腈,梯度洗脱;电喷雾离子源,正离子提取离子模式。结果马兜铃果皮和种子中5种成分含量及总含量差异明显,种子中5种成分总含量约为果皮中的50倍。结论马兜铃存在区分部位来用药的可能性,以果皮入药更安全。

关 键 词：马兜铃 马兜铃酸 马兜铃内酰胺 UPLC-MS 

分 类 号：R284.1[医药卫生—中药学]