维甲酸对成肌细胞细胞周期进程及凋亡的影响  

Effects of all-trans retinoic acid on cell cycle progression and apoptosis of C2C12 mouse myoblasts

作　　者：李成绪 马莉[1] 连帅[1] 徐彬 逯静静 李鑫月 李倩茹 杨焕民[1] LI Chengxu;MA Li;LIAN Shuai;XU Bin;LU Jingjing;LI Xinyue;LI Qianru;YANG Huanmin(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163000,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163000

出　　处：《中国兽医学报》2021年第10期1998-2002,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31672513)。

摘　　要：成肌细胞的周期进程及凋亡对骨骼肌的损伤修复至关重要。本研究旨在细胞水平探讨全反式维甲酸(ATRA)对C2C12细胞周期进程及凋亡的影响。试验组C2C12细胞用100 nmol/L ATRA处理48 h,对照组不予处理。用MTS、Ki67及EdU法检测ATRA对C2C12细胞增殖的影响,通过Western blot检测ATRA对C2C12细胞中增殖相关蛋白CDK2、CDK4、CDK6以及细胞周期蛋白D1(Ccnd1)、Ccnd2、Ccnd3和Ccne1表达的影响,并检测ATRA对Bcl-2、Bax、Caspase-3等凋亡相关蛋白表达的影响,最后通过流式细胞术检测细胞周期进程以及凋亡率。结果显示,与对照组相比,100 nmol/L ATRA可促进C2C12细胞增殖,并且上调上述几种增殖相关蛋白的表达水平。ATRA促进C2C12细胞从G1期进入S期。此外,ATRA处理使C2C12细胞Bcl-2显著升高,而Bax及活化的Caspase-3显著降低,从而显著降低了细胞凋亡率。综上所述,100 nmol/L ATRA处理48 h会促进C2C12细胞的增殖并抑制细胞凋亡,对骨骼肌的损伤修复具有潜在作用。The proliferation and apoptosis of myoblasts are crucial to the repair of skeletal muscle damage.This study aims to investigate the mechanism of all-trans retinoic acid(ATRA)on the proliferation and apoptosis of C2 C12 cells.C2 C12 cells in the experimental group were treated with 100 nmol/L ATRA for 48 h,while the control group was not treated.MTS,Ki67 and EdU methods were used to detect the effect of ATRA on the proliferation of C2 C12 cells.Western blot was used to detect the effects of ATRA on the expression of CDK2,CDK4,and CDK6,cyclin D1(Ccnd1),Ccnd2,Ccnd3 and Ccne1 of C2 C12 cells,as well as several apoptosis related proteins,including Bcl-2,Bax,caspase-3.Cell cycle progression and apoptosis rate were detected by flow cytometry.The results showed that 100 nmol/L ATRA can promote the proliferation of C2 C12 cells and enhance the expression of cyclins Ccnd1,Ccnd2,Ccnd3,Ccne1,CDK2,CDK4 and CDK6 compared with the control group.Flow cytometry results showed that ATRA promotes C2 C12 cells from G1 phase to S phase.In addition,ATRA treatment significantly increased Bcl-2 and decreased Bax and activated Caspase-3 in C2 C12 cells,resulting in a significant decrease in apoptosis.In summary,our results indicate that 100 nmol/L ATRA treatment for 48 h can promote the proliferation of C2 C12 cells and inhibit apoptosis,which has a potential effect on the repair of skeletal muscle damage.

关 键 词：全反式维甲酸(ATRA) 细胞凋亡 C2C12细胞 周期蛋白 

分 类 号：Q253[生物学—细胞生物学] Q255