宫颈鳞状细胞癌组织中相关蛋白表达与临床病理特征上皮间质转化及预后的关系  

Correlation between expression of related proteins and clinicopathologic features,epithelial-mesenchymal transformation and prognosis in cervical squamous cell carcinoma

作　　者：戴丹 黄炎[1] 春芽 DAI Dan;HUANG Yan;CHUN Ya(Department of Pathology,the First Peopled Hospital of Yuha ng District,Hangzhou,Zhejiang 311100,China)

机构地区：[1]杭州市余杭区第一人民医院病理科,浙江杭州311100 [2]宝鸡市中医医院妇科,陕西宝鸡721000

出　　处：《中国妇幼保健》2021年第21期5043-5047,共5页Maternal and Child Health Care of China

基　　金：浙江省医药卫生科技计划项目(2013KY045)。

摘　　要：目的研究宫颈鳞状细胞癌组织中高尔基体膜蛋白1(GOLM1)、肿瘤坏死因子-α诱导蛋白8样蛋白3(TIPE3)蛋白表达与临床病理特征、上皮间质转化(EMT)及预后的关系,为临床宫颈鳞状细胞癌的防治提供新的靶点和思路。方法选取2013年1月-2016年1月杭州市余杭区第一人民医院收治的宫颈鳞状细胞癌患者80例为研究对象。分别采集宫颈鳞状细胞癌组织和癌旁正常组织,检测并比较两种组织中的GOLM1、TIPE3蛋白及EMT相关蛋白表达情况。分析GOLM1、TIPE3蛋白与宫颈鳞状细胞癌临床病理特征、EMT相关蛋白及预后的关系。多因素Cox回归分析宫颈鳞状细胞癌患者预后的影响因素。结果宫颈鳞状细胞癌组织中GOLM1、TIPE3及Vimentin蛋白阳性表达率(72.50%、71.25%及68.75%)明显高于癌旁正常组织(17.50%、15.00%及36.25%),而E-cadherin蛋白阳性表达率(28.75%)明显低于癌旁正常组织(63.75%),差异均有统计学意义(χ^(2)=48.889,P<0.05;χ^(2)=51.601,P<0.05;χ^(2)=16.942,P<0.05;χ^(2)=19.711,P<0.05)。宫颈鳞状细胞癌组织中GOLM1、TIPE3蛋白表达与E-cadherin蛋白表达呈负相关关系(r=-0.441,P<0.05;r=-0.409,P<0.05),与Vimentin蛋白表达呈正相关关系(r=0.472、0.453,均P<0.05)。低分化、FIGO分期Ⅱ~Ⅲ期及淋巴结转移宫颈鳞状细胞癌患者的GOLM1(93.33%、89.80%及92.86%)、TIPE3蛋白(93.99%、85.71%及85.71%)阳性表达率高于中高分化、FIGO分期Ⅰ期及无淋巴结转移患者(67.69%、45.16%、61.54%,66.15%、48.39%、63.46%),差异均有统计学意义(χ^(2)=4.019,P<0.05;χ^(2)=18.973,P<0.05;χ^(2)=8.954,P<0.05;χ^(2)=4.395,P<0.05;χ^(2)=12.915,P<0.05;χ^(2)=4.400,P<0.05)。随访期间GOLM1阳性患者死亡率(68.97%)高于GOLM1阴性患者(31.82%),且TIPE3阳性患者死亡率(73.68%)高于GOLM1阴性患者(21.74%),差异均有统计学意义(χ^(2)=6.514,P<0.05;χ^(2)=6.733,P<0.05)。多因素Cox回归分析结果显示,低分化、FIGO分期、淋巴结转移、GOLM1蛋白阳性及TIPObjective To investigate the relationship between GOLM1,tumor necrosis factor-αinduced protein 8-like protein-3(TIPE3)and clinicopathologic features,epithelial-mesenchymal transition(EMT)and prognosis in cervical squamous cell carcinoma,provide a new target and ideas for clinical prevention and treatment of cervical squamous cell carcinoma.Methods A total of 80 patients with cervical squamous cell carcinoma admitted to the First People’s Hospital of Yuhang District,Hangzhou from January 2013 to January 2016 were selected as the study subjects.The expression of GOLM1,TIPE3 and EMT-related proteins in cervical squamous cell carcinoma tissues and paracancer normal tissues were detected and compared.To analyze the relationship between GOLM1,TIPE3 and clinicopathologic features,EMT-related proteins and prognosis of cervical squamous cell carcinoma.Multivariate cox regression analysis of prognostic factors in patients with cervical squamous cell carcinoma.Results The positive expression rate of GOLM1,TIPE3 and Vimentin in cervical squamous cell carcinoma tissues(72.50%,71.25%,68.75%)were significantly higher than that in adjacent normal tissues(17.50%,15.00%,36.25%),while the positive expression rate of E-cadherin protein(28.75%)was significantly lower than that in adjacent normal tissue(63.75%),with statistical significance(χ^(2)=48.889,P<0.05;χ^(2)=51.601,P<0.05;χ^(2)=16.942,P<0.05;χ^(2)=19.711,P<0.05).The protein expression of GOLM1 and TIPE3 were negatively correlated with the protein expression of e-cadherin(r=-0.441,P<0.05;r=-0.409,P<0.05),and positively correlated with the protein expression of Vimentin(r=0.472,0.453,all P<0.05)in cervical squamous cell carcinoma.The positive expression rates of GOLM1(93.33%,89.80%,92.86%)and TIPE3(93.99%,85.71%,85.71%)protein in patients with low-differentiation,FIGO stageⅡ-Ⅲand lymph node metastasis were higher than those in patients with medium-high differentiation,FIGO stageⅠand no lymph node metastasis(67.69%,45.16%,61.54%,66.15%,48.39%,63.46%),with statistical

关 键 词：宫颈鳞状细胞癌 高尔基体膜蛋白1 肿瘤坏死因子-α诱导蛋白8样蛋白3 上皮间质转化 预后 

分 类 号：R711.74[医药卫生—妇产科学]