越南槐根茎中的生物碱成分及其抗炎活性  

Alkaloids from the roots and rhizomes of Sophora tonkinensis and its anti-inflammatory activities

作　　者：张袁亚 张治军 叶瑞绒 李蓉涛[1] ZHANG Yuan-ya;ZHANG Zhi-jun;YE Rui-rong;LI Rong-tao(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《药学学报》2021年第10期2825-2829,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31960092,22067012,82073737);云南省创新团队资助项目(2019HC018)。

摘　　要：采用多种色谱技术从越南槐根茎中分离得到了25个喹诺里西啶类生物碱(包括苦参碱型1~14、鹰爪豆碱型15~17、金雀花碱型18~23、其他型24和25),并依据理化性质及NMR、MS波谱数据鉴定了化合物的结构。其中12-(1-乙酰氧基乙基)-金雀花碱(23)为新的金雀花碱型衍生物,化合物13、16、17和24为首次从越南槐根茎中分离得到。化合物1、6、19和20可明显抑制脂多糖(LPS)诱导小鼠巨噬细胞RAW 264.7释放NO,IC50值分别为39.86±0.65、23.66±0.37、34.56±0.45、47.68±0.58μmol·L^(-1)。Twenty-five quinolizidine alkaloids(including matrine-type 1-14,sparteine-type 15-17,cytisinetype 18-23,other types 24 and 25)were isolated from the roots and rhizomes of Sophora tonkinensis by various chromatographic methods.Their structures were elucidated by physicochemical properties,NMR and MS spectral data.Among them,12-(1-acetoxyethyl)-cytisine(23)is a new alkaloid derivative,and compounds 13,16,17,24 were isolated from the roots and rhizomes of S.tonkinensis for the first time.Compounds 1,6,19 and 20 showed potent inhibitory activity against LPS-induced NO production in RAW 264.7 macrophages,with IC50 values of 39.86±0.65,23.66±0.37,34.56±0.45,47.68±0.58μmol·L^(-1),respectively.

关 键 词：越南槐 喹诺里西啶类生物碱 12-(1-乙酰氧基乙基)-金雀花碱 抗炎活性 

分 类 号：R284[医药卫生—中药学]