高效液相色谱法同时测定茵栀黄制剂中4种有效成分含量  

Simultaneous Determination of Four Active Ingredients in Yinzhihuang Preparations by HPLC

作　　者：张伟[1] 孙亦钊 张娟娟[1] 黄妍妍 王世林 陈林[1] 尹震花[1] ZHANG Wei;SUN Yizhao;ZHANG Juanjuan;HUANG Yanyan;WANG Shilin;Chen Lin;YIN Zhenhua(Huanghe Science and Technology College•Zhengzhou Key Laboratory of Synthetic Biology of Natural Products,Zhengzhou,Henan,China 45006)

机构地区：[1]黄河科技学院·郑州市天然产物合成生物学重点实验室,河南郑州450063

出　　处：《中国药业》2021年第21期73-76,共4页China Pharmaceuticals

基　　金：河南省科技发展计划项目[182107000033];河南省郑州市基础研究及应用基础研究专项资金项目[ZZSZX202003];河南省大学生创新创业训练计划项目[S202011834013];黄河科技学院大学生创新创业项目[2020XSCXCY103]。

摘　　要：目的建立同时测定茵栀黄颗粒、口服液、胶囊中绿原酸、黄芩苷、栀子苷、木犀草素4种有效成分含量的高效液相色谱法。方法色谱柱为Xterra MS C18柱(250 mm×4.6 mm,5μm),流动相为0.1%甲酸溶液(A)-乙腈(B),梯度洗脱(0~10 min时10%B~30%B,10~25 min时30%B),流速为1.0 mL/min,检测波长为254 nm,柱温为30℃,进样量为10μL。结果茵栀黄颗粒、口服液、胶囊中绿原酸、黄芩苷、栀子苷、木犀草素进样量分别在0.012 65~0.303 6 0μg、0.808~6.464μg、0.063 12~0.378 72μg、0.005 33~0.106 50μg范围内与峰面积线性关系良好(r> 0.999 0),检测限分别为0.12,2.64,0.18,0.33 ng,定量限分别为0.27,7.66,0.42,0.81 ng,精密度、重复性、稳定性试验的RSD均小于3.0%(n=6),平均回收率分别为99.46%,98.86%,98.60%,99.25%, RSD分别为0.94%,1.40%,1.30%,0.89%(n=6)。绿原酸、黄芩苷、栀子苷、木犀草素的含量,茵栀黄颗粒中分别为3.661,77.043,3.102,0.760 mg/g,茵栀黄胶囊中分别为27.859,780.433,15.030,4.589 mg/g,茵栀黄口服液中分别为0.837,53.998,2.047,0.510 mg/mL。结论该方法操作简便,灵敏度高,重复性好,稳定可靠,可用于同时测定茵栀黄制剂中绿原酸、黄芩苷、栀子苷、木犀草素的含量。Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of chlorogenic acid,baicalin,geniposide and luteolin in Yinzhihuang Granule,Oral Liquid and Capsule.Methods The chromatographic column was Xterra MS C18 column(250 mm×4.6 mm,5μm),the mobile phase was 0.1%formic acid solution(A)-acetonitrile(B)with gradient elution(0-10 min,10%B-30%B;10-25 min,30%B),the flow rate was 1.0 mL/min,the detection wavelength was 254 nm,the column temperature was 30℃,and the injection volume was 10μL.Results The chlorogenic acid,baicalin,geniposide and luteolin in Yinzhihuang Granule,Oral Liquid and Capsule showed a linear relationship in the ranges of 0.01265-0.30360μg,0.808-6.464μg,0.06312-0.37872μg,0.00533-0.10650μg(r>0.9990),respectively.Their limits of detection were 0.12,2.64,0.18 and0.33 ng,respectively,and the limits of quantification were 0.27,7.66,0.42,and 0.81 ng,respectively.The RSDs of precision,repeatability and stability tests were less than 3.0%(n=6).The average recoveries of chlorogenic acid,baicalin,geniposide and luteolin were99.46%,98.86%,98.60%and 99.25%with RSDs of 0.94%,1.40%,1.30%and 0.89%(n=6),respectively.The contents of chlorogenic acid,baicalin,geniposide and luteolin in Yinzhihuang Granule were 3.661,77.043,3.102 and 0.760 mg/g,respectively.The contents of the above four active ingredientc in Yinzhihuang Capsule were 27.859,780.433,15.030 and 4.589 mg/g,respectively.The contents of the above four active ingredientc in Yinzhihuang Oral Liquid were 0.837,53.998,2.047 and 0.510 mg/g,respectively.Conclusion The method is simple,sensitive,reproducible,stable and reliable,which can be used for the simultaneous determination of chlorogenic acid,baicalin,geniposide and luteolin in Yinzhihuang preparations.

关 键 词：茵栀黄制剂 高效液相色谱法 绿原酸 黄芩苷 栀子苷 木犀草素 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]