盘龙七片对椎动脉型颈椎病大鼠的干预作用  

Effect of Panlongqi Tablet on Cervical Spondylosis of Vertebral Artery Type in Rats

作　　者：明瑞蕊 徐腾腾 王金霞 贾可欣 刘春芳[2] 陈卫衡 林娜[1,2] MING Rui-rui;XU Teng-teng;WANG Jin-xia;JIA Ke-xin;LIU Chun-fang;CHEN Wei-heng;LIN Na(Chengde Medical University,Chengde 067000,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]承德医学院,河北承德067000 [2]中国中医科学院中药研究所,北京100700 [3]北京中医药大学第三附属医院,北京100029

出　　处：《中国实验方剂学杂志》2021年第22期51-58,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院科技创新工程项目(C12021A03808);中国中医科学院中药研究所技术研发项目(20191306);国家“重大新药创制”科技重大专项(2019ZX09731002)。

摘　　要：目的:采用混合造模方法建立椎动脉型颈椎病(CSA)大鼠模型,观察盘龙七片(PLQT)对CSA大鼠的干预作用。方法:SD大鼠,设立正常,模型,PLQT(0.16,0.32,0.64 g·kg^(-1)),阳性药颈复康颗粒(JFK,1.35 g·kg^(-1))组,于最后一次注射完造模剂24 h后开始给药,模型组灌服等体积生理盐水,持续给药8周。斜板实验检测肢体运动功能、小动物多模高频声波检测椎动脉血流速度、激光多普勒检测软脑膜微循环血流量、微计算机断层扫描(Micro CT)检测颈椎影像学改变程度并进行评分,酶联免疫吸附测定(ELISA)检测血清中血清内皮素-1(ET-1),一氧化氮(NO),组织纤维溶酶原激活物(t-PA),纤溶酶原激活物抑制物(PAI)的含量。结果:与正常组比较,CSA模型组大鼠肢体运动功能、椎动脉血流速度、软脑膜微循环血流量显著降低,颈椎影像学改变程度和评分明显升高(P<0.05,P<0.01),PLQT作用后可剂量依赖性地改善CSA大鼠的运动功能、升高椎动脉血流速度和软脑膜微循环血流量,并明显降低颈椎影像学改变程度和评分(P<0.05,P<0.01);CSA模型组大鼠较正常组明显下调血清中NO和t-PA含量,升高ET-1,PAI含量,PLQT作用后明显升高血清中NO,t-PA含量,明显降低ET-1,PAI含量(P<0.05,P<0.01)。结论:PLQT能改善CSA大鼠的肢体运动功能、促进椎动脉和软脑膜微循环的血流量、改善颈椎影像学改变程度,调节血管舒缩功能和改善凝血纤溶系统,具有较好的治疗CSA的潜能。Objective: To establish a model of cervical spondylosis of vertebral artery type(CSA)in rats by mixed modeling method,and observe the intervention effect of Panlongqi tablet(PLQT)on CSA rats.Method: SD rats were divided into a normal control group,a model group,low-(0.16 g·kg^(-1)),medium-(0.32 g·kg^(-1)),and high-dose(0.64 g·kg^(-1))PLQT groups,and a Jingfukang granule(JFK,1.35 g·kg^(-1))group.The rats were treated correspondingly 24 hours after modeling for eight weeks,and those in the normal control group received an equal volume of normal saline by gavage. The limb movement was tested by the inclined plate assay, vertebral artery flow volume by multi-mode high-frequency sound wave for small animals, and microcirculatory blood flow in the pia mater by the laser Doppler. The imaging of the cervical spine was recorded and scored by X-ray micro-computed tomography(Micro CT). Enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of endothelin-1(ET-1),nitric oxide(NO),tissue plasminogen activator(tPA),and plasminogen activator inhibitor(PAI). Result: Compared with the normal control group,the model group showed decreased limb movement,vertebral artery flow volume,and microcirculatory blood flow in the pia mater, and increased imaging of the cervical spine and score(P<0.05,P<0.01). PLQT could dosedependently improve the motor function,increase the vertebral artery flow volume and microcirculatory blood flow in the pia mater,and reduce the degree and score of imaging of the cervical spine in CSA rats(P<0.05,P<0.01). The serum levels of NO and t-PA were decreased and those of ET-1 and PAI were increased in the model group as compared with those in the normal control group,while such changes were reversed by PLQT treatment(P<0.05,P<0.01). Conclusion: PLQT can enhance the limb movement,promote the vertebral artery flow volume and microcirculatory blood flow in the pia mater,improve the degree of imaging of the cervical spine,regulate the vasomotor function,and improve the coagulation and fibrinolys

关 键 词：盘龙七片 大鼠 椎动脉型颈椎病 椎动脉血流 血管舒缩功能 

分 类 号：R2-0[医药卫生—中医学] R289