胎膜早破孕妇外周血CD8^(+)CD25^(+)FoxP3^(+)调节性T细胞表达水平对辅助性T细胞1/辅助性T细胞2平衡的影响  

The effect of CD8^(+)CD25^(+)FoxP3^(+) regulatory T cell expression levels on balance of helper T cells 1/helper T cells 2 in peripheral blood of pregnant women with premature rupture of fetal membranes

作　　者：汪洪友[1] 周建波[1] 于蕾[1] 王永芳[1] Wang Hongyou;Zhou Jianbo;Yu Lei;Wang Yongfang(Department of Gynaecology and Obstetrics,Binhai County People′s Hospital,Jiangsu Yancheng 224500,China)

机构地区：[1]江苏省滨海县人民医院妇产科,224500

出　　处：《中国医师进修杂志》2021年第10期926-930,共5页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨胎膜早破(PROM)孕妇外周血CD8^(+)CD25^(+)FoxP3^(+)调节性T细胞(Treg)表达水平对辅助性T细胞(Th)1/Th2平衡的影响。方法选择2019年9月至2020年5月江苏省滨海县人民医院收治的PROM孕妇(PROM组)、正常足月妊娠孕妇(正常妊娠组)及正常未孕女性(未孕组)各30例为研究对象,取各组外周血,流式细胞仪测定CD8^(+)CD25^(+)FoxP3^(+)Treg比例;提取外周血单个核细胞(PBMCs),聚合酶链反应法(PCR)测定叉头翼状螺旋转录因子3(FoxP3)mRNA;Luminex液相芯片法测定Th1相关细胞因子干扰素-γ(IFN-γ)、白细胞介素(IL)-2及Th2相关细胞因子IL-10、IL-4水平;分析CD8^(+)CD25^(+)FoxP3^(+)Treg表达对Th1/Th2平衡的影响。结果PROM组、正常妊娠组CD8^(+)CD25^(+)FoxP3^(+)Treg比例及FoxP3 mRNA表达水平低于未孕组[(0.15±0.03)%和(0.35±0.09)%比(0.47±0.11)%、0.89±0.11和3.15±0.67比3.75±0.23],PROM组CD8^(+)CD25^(+)FoxP3^(+)Treg比例及FoxP3 mRNA表达水平又低于正常妊娠组,差异均有统计学意义(P<0.05)。PROM组、正常妊娠组IFN-γ、IL-2水平高于未孕组,IL-4水平低于未孕组,PROM组IFN-γ、IL-2水平又高于正常妊娠组,IL-4水平又低于正常妊娠组,差异均有统计学意义(P<0.05)。PROM组孕妇外周血CD8^(+)CD25^(+)FoxP3^(+)Treg比例及FoxP3 mRNA表达水平与IFN-γ(r=-0.413、-0.451,P<0.05)、IL-2(r=-0.645、-0.535,P<0.05)呈负相关,与IL-4水平呈正相关(r=0.558、0.469,P<0.05)。结论PROM孕妇外周血CD8^(+)CD25^(+)FoxP3^(+)Treg比例较低,FoxP3 mRNA表达水平较低,均影响Th1/Th2免疫平衡,引起Th1免疫漂移,可能为PROM发生的相关免疫机制。Objective To investigate the effect of CD8^(+)CD25^(+)FoxP3^(+)regulatory T cell(Treg)expression levels in peripheral blood of pregnant women with premature rupture of fetal membranes(PROM)on immune function of helper T cells(Th)1/Th2.Methods Thirty cases of pregnant women with PROM(PROM group),30 cases of normal pregnant women(normal pregnancy group)and 30 cases of normal non-pregnant women(non-pregnancy group)who treated in Binhai County People′s Hospital from September 2019 to May 2020 were collected.Peripheral blood of each group was collected and the proportion of CD8^(+)CD25^(+)FoxP3^(+)Treg was determined by flow cytometry.Peripheral blood mononuclear cells(PBMCs)were extracted and FoxP3 mRNA was determined by polymerase chain reaction(PCR).The levels of Th1-related cytokines interferon-γ(IFN-γ),interleukin(IL)-2,and Th2-related cytokines IL-10 and IL-4 were measured by Luminex liquid phase microarray.The effects of CD8^(+)CD25^(+)FoxP3^(+)Tregexpression on Th1/Th2 balance were analyzed.Results The proportion of CD8^(+)CD25^(+)FoxP3^(+)Tregand the expression of FoxP3 mRNA in PROM groupand normal pregnancy group were lower than those in non-pregnancy group:(0.15±0.03)%,(0.35±0.09)%vs.(0.47±0.11)%;0.89±0.11,3.15±0.67 vs.3.75±0.23,the proportion of CD8^(+)CD25^(+)FoxP3^(+)Treg and the expression of FoxP3 mRNA in PROM groupwere lower than those in the normal pregnancy group,and the differences were statistically significant(P<0.05).The levels of Th1-related cytokines IFN-γand IL-2 in PROM group and normal pregnancy group were higher than those in non-pregnancy group,the level of Th2-related cytokines IL-4 was lower than that in non-pregnancy group,the levels of IFN-γand IL-2 in PROM group were higher than those in normal pregnancy group,the level of IL-4 was lower than that in normal pregnancy group,and the differences were statistically significant(P<0.05).In PROM group,the proportion of CD8^(+)CD25^(+)FoxP3^(+)Treg and the expression of FoxP3 mRNA in peripheral blood were negatively correlated wi

关 键 词：胎膜早破 T淋巴细胞 辅助诱导 免疫 细胞 

分 类 号：R714.433[医药卫生—妇产科学]