脂肪干细胞对中长波紫外线损伤小鼠皮肤的修复研究  

Repair of UVB damage in mouse skin by adipose derived stem cells

作　　者：吕安琪 徐二伟 单士军 王琪影[3] Lyu Anqi;Xu Erwei;Shan Shijun;Wang Qiying(Department of Dermatology,Xiang'an Hospital of Xiamen University,Xiamen 361000,China;Xiamen Funano Material Technology Co.,Ltd,Xiamen 361000,China;Department of Plastic Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]厦门大学附属翔安医院皮肤科,厦门361000 [2]厦门福纳新材料科技有限公司,厦门361000 [3]郑州大学第一附属医院整形外科,郑州450000

出　　处：《中华医学美学美容杂志》2021年第5期436-439,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：河南省高等学校重点科研项目(19B320029)。

摘　　要：目的探讨脂肪干细胞(ADSC)对小鼠皮肤中长波紫外线损伤的修复效果,为皮肤光损伤治疗提供思路。方法2018年8月至2019年7月,郑州大学第一附属医院整形外科提取C57BL/6小鼠腹股沟及肾周脂肪组织,处理获得小鼠ADSC,并进行表面标志物、成脂、成骨分化能力鉴定。小鼠光老化模型用SS-03AB型紫外光照仪照射,UVB总剂量9.45 J/cm^(2),UVA总剂量94.5 J/cm^(2)。实验小鼠(72只)分为正常组、模型组、DMEM(培养基)组、ADSC组,各18只。正常组、模型组照射结束饲养2周后取材,ADSC组照射结束后给予200μl ADSC悬液皮下注射,DMEM组给予200μl无血清培养基,均在2周后取材进行组织病理学染色。实验数据采用方差分析处理。结果提取细胞鉴定为脂肪干细胞。HE染色可见镜下炎症细胞浸润ADSC组比DMEM组(t=20.649,P<0.001)和正常组(t=16.147,P<0.001)显著减轻,真皮层厚度增加。Masson染色见ADSC治疗后胶原纤维排列整齐密度明显增高。结论皮下注射ADSC可减轻炎症反应,促胶原组织增生,增加真皮层厚度,有效抵抗UVB造成的炎症损伤和胶原断裂。Objective To explore the therapeutic effect of adipose-derived stem cells(ADSC)on long-wave UV damage in mouse skin in order to provide ideas for the treatment of skin photodamage.Methods The inguinal and perirenal adipose tissues of C57BL/6 mice were extracted and processed to obtain mouse ADSCs,and the surface markers,adipogenic and osteogenic differentiation capabilities were identified.The mouse photoaging model was irradiated with the SS-03AB UV illuminator,the total UVB dose was 9.45 J/cm^(2),and the total UVA dose was 94.5 J/cm^(2).Experimental mice(72 in total)were divided into normal group,model group,DMEM(medium)group and ADSC group,each with 18 mice.In the normal group and model group,the materials were taken two weeks after the end of irradiation.After irradiation,the ADSC group was given a subcutaneous injection of 200μl ADSC suspension,and the DMEM group was given 200μl of serum-free medium for treatment,and the materials were taken for pathological staining after 2 weeks.The experimental data was processed by analysis of variance.This study was carried out from August 2018 to July 2019 in the First Affiliated Hospital of Zhengzhou University.Results The extracted cells were identified as adipose-derived stem cells.HE staining showed that the inflammatory cell infiltration in the ADSC group was significantly reduced compared with the DMEM group(t=20.649,P<0.001)and the normal group(t=16.147,P<0.001),and the thickness of the dermis layer was significantly increased.Masson staining showed collagen fibers were arranged neatly and the density increased significantly after ADSC treatment.Conclusions Subcutaneous injection of ADSC can reduce inflammation,promote collagen tissue proliferation,increase the thickness of the dermis,effectively resist inflammatory damage and collagen breakdown caused by UVB.

关 键 词：小鼠 炎症 脂肪干细胞 光老化 光损伤 胶原纤维 

分 类 号：R622[医药卫生—整形外科]