黄瓜籽蛋白对颈总动脉血栓模型大鼠血清TXB_(2)6-keto-PGF1α和T-PA的调节作用  

Regulatory Effects of Cucumber Seeds Protein on Serum TXB_(2),6-keto-PGF1αand T-PA in Rats with Carotid Artery Thrombosis

作　　者：王萍 陈丽艳 孙银玲 姚琳 董坤 高辛 王伟明 WANG Ping;CHEN Liyan;SUN Yinling;YAO Lin;DONG Kun;GAO Xin;WANG Weiming(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中国中医药科技》2021年第6期901-903,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：现代农业产业技术体系建设专项资金资助项目(CARS-21);黑龙江省应用技术研究与开发计划项目(GA17C017)。

摘　　要：目的:探讨黄瓜籽蛋白对颈总动脉血栓模型大鼠纤溶凝血系统功能的影响。方法:50只大鼠随机分为模型组、阳性对照组(蚓激酶,25000 IU·kg-1·d-1)和黄瓜籽蛋白低、中、高剂量组(5000、25000、125000 IU·kg-1·d-1),每组10只。采用FeCl 3诱导大鼠颈总动脉血栓模型,各组给予相应药物14 d,ELISA测定大鼠血清血栓素B 2(TXB_(2))、6-酮-前列腺素F1α(6-keto-PGF1α)和纤溶酶原活化剂(T-PA)含量。结果:与模型组比较,黄瓜籽蛋白低、中、高剂量组大鼠TXB 2含量减少,其中高剂量组减少明显(P<0.01);黄瓜籽蛋白低、中、高剂量组大鼠6-keto-PGF1α含量明显增加,其中中剂量组增加明显(P<0.01);黄瓜籽蛋白低、中、高剂量组大鼠T-PA含量明显增加,其中高剂量组增加明显(P<0.01)。结论:黄瓜籽蛋白对颈总动脉血栓大鼠血清TXB 2、6-keto-PGF1α、T-PA具有一定的调节作用。Objective:To investigate the effects of Cucumber seeds protein(CSP)on the function of fibrinolytic coagulation system in rats with carotid artery thrombosis.Methods:50 rats were divided in model,positive(Lumbrokinase,25000 IU·kg-1·d-1)and CSP low,middel,high dose(5000、25000、125000 IU·kg-1·d-1)groups with each10 rats.FeCl 3 was applied to induce the thrombosis formation in rat’s common carotid artery,each group was given the corresponding medicine for 14 days,then rats’serum thromboxane B 2(TXB_(2)),6-keto-prostaglandin F1α(6-keto-PGF1α)and tissue type plasminogen activator(t-PA)were determined by ELISA.Results:To compare with model group rats,The TXB_(2) content was significantly decreased in three different dosages of CSP groups and especially in high dosage CSP(P<0.01),The 6-keto-PGF1αcontent was significantly increased in three different dosages of CSP groups and especially in middle dosage CSP(P<0.01),The T-PA content was significantly increased in three different dosages of CSP groups and especially in high dosage CSP(P<0.01).Conclusion:CSP can regulate serum TXB 2,6-keto-PGF1αand t-PA of rats with carotid artery thrombosis.

关 键 词：黄瓜籽蛋白 颈总动脉血栓 血栓素B_(2) 6-酮-前列腺素 纤溶酶原活化剂 大鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]