厚朴酚对H1N1流感病毒性肺炎防治作用的实验研究  

Intervention Effects of Magnolol on Viral Pneumonia Caused by Influenza H1N1

作　　者：周陈建 赵嫏嬛 吴晓宁[3] ZHOU Chenjian;ZHAO Langhuan;WU Xiaoning(Wenzhou Central Hospital,Wenzhou,Zhejiang 32500;Wenzhou People’s Hospital,Wenzhou,Zhejiang 32500;Hangzhou Medical College,Hangzhou,Zhejiang310053)

机构地区：[1]温州市中心医院药剂科,浙江温州32500 [2]温州市人民医院,浙江温325000 [3]杭州医学院,浙江杭州310053

出　　处：《中国中医药科技》2021年第6期903-905,F0003,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省温州市基础性医疗卫生科技项目(Y20190704)。

摘　　要：目的:探讨厚朴酚对H1N1所致流感病毒性肺炎的防治作用及其作用机制。方法:60只小鼠随机分成正常对照组、模型组、厚朴酚低剂量组(96 mg/kg)、中剂量组(192 mg/kg)、高剂量组(384 mg/kg)和阳性(利巴韦林,180 mg/kg)组,每组10只。通过流感病毒鼻腔攻毒建立感染小鼠病毒性肺炎模型,同时灌胃相应药物或纯净水;采用HE染色观察各组小鼠肺组织肺泡的保护作用;采用Western Blot法检测各组小鼠肺组织Toll样受体7(TLR7)、细胞内髓样分化因子88(MyD88)、核因子-κBp65(NF-κBp65)、肿瘤坏死因子-α(TNF-α)蛋白表达的变化。结果:厚朴酚各剂量组均能不同程度减轻肺组织肺泡损伤,降低TLR7、MyD88、NF-κBp65、TNF-α的表达。结论:厚朴酚能抑制流感(H1N1)所致病毒性肺炎的炎症反应。Objective:To investigate the intervention effect of Magnolol on viral pneumonia caused by influenza(H1N1)and its mechanism.Methods:60 mice were divided into normal,model,low,middle,high dose Magnolol(96,192,384 mg/kg)and positive(Ribavirin,180 mg/kg)groups with each 10 mice.The model of viral pneumonia was established by nasal challenge of influenza virus,and Magnolol,Ribavirin and purified water was given the corresponding group respectively by gavage.The method of hematoxylin eosin staining was used to investigate the protective effect of each group on pulmonary alveoli.Western blot was used to analyse the protein levels of TLR7 MyD88 NF-κBp65 and TNF-α.Results:Magnolol can reduce the alveolar injury of lung tissue in different degrees,Magnolol with in 96~384 mg/kg could suppress the levels of TLR7 MyD88 NF-κBp65 and TNF-α.Conclusion:Magnolol can inhibit the inflammatory reaction of viral pneumonia caused by influenza(H1N1).

关 键 词：厚朴酚 流感(H1N1) 肺炎 病理学 TOLL样受体信号通路 小鼠 

分 类 号：R285.5[医药卫生—中药学]