Characterization and expression analysis of genes encoding Taxol biosynthetic enzymes in Taxus spp.  

作　　者：Luyuan Jiang Kaikai Zhang Xing Lv Liying Yang Shuai Wang Duanfen Chen Yanfang Yang Deyou Qiu 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China [2]College of Horticulture,Hebei Agricultural University,Baoding 071001,People’s Republic of China [3]College of Agronomy,Hebei Agricultural University,Baoding 071001,People’s Republic of China [4]School of Pharmacy,Liaocheng University,Liaocheng 252000,People’s Republic of China

出　　处：《Journal of Forestry Research》2021年第6期2507-2515,共9页林业研究（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(31570675);a Grant from the National Key Research and Development Program of China(2017YFD060070605);a Grant for National non-profit Research Institutions of Chinese Academy of Forestry(CAFYBB2018SY009).

摘　　要：Taxol(Paclitaxel),an important anticancer drug,is derived at very low yields from Taxus(yew)species that grow very slowly.In the present study,thirteen genes that encode enzymes involved in Taxol biosynthesis in Taxus spp.were analyzed with bioinformatics methods,and their expression levels in different tissues and after cold and hormone treatments were also analyzed.The results indicated that many cis-elements related to abiotic stresses and hormones were found in the promoter sequences of the 8 genes involved in Taxol biosynthesis.Moreover,the 13 enzymes encoded by the target genes were located in different organelles and had many phosphorylation sites in the response proteins.The 13 genes were expressed highly either in roots or in stems,with lower transcripts in needles,and they were highly expressed after treatment with cold,gibberellin,methyl jasmonate or coronatine,consistent with predictions based on the bioinformatics analysis.These results suggest that the factors such as hormones and abiotic stresses stimulate taxane biosynthesis in yews,providing an important way to sustainably generate taxanes from yew trees or their cell cultures to improve Taxol yields.

关 键 词：Gene expression Bioinformatics analysis Acyltransferase Cytochrome P450 Abiotic stress Hormone 

分 类 号：S791.49[农业科学—林木遗传育种]