烟草白粉病抗性连锁分子标记开发及育种利用  

Development and breeding utilization of molecular markers linked to powdery mildew resistance in tobacco

作　　者：张孝廉 张吉顺 贾蒙骜 张莉莉 曹领改 ZHANG Xiaolian;ZHANG Jishun;JIA Mengao;ZHANG Lili;CAO Linggai(Molecular Genetics Key Laboratory of China Tobacco,Guizhou Academy of Tobacco Science,Guiyang 550081,China;College of Life Sciences,Guizhou University,Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guiyang 550025,China)

机构地区：[1]贵州省烟草科学研究院,烟草行业分子遗传重点实验室,贵阳市550081 [2]贵州大学生命科学学院,贵州省农业生物工程重点实验室,山地植物资源保护与种质创新省部共建教育部重点实验室,贵阳550025

出　　处：《中国烟草学报》2021年第5期45-51,共7页Acta Tabacaria Sinica

基　　金：中国烟草总公司贵州省公司科技项目“烟草白粉病和PVY抗性基因聚合及育种材料创制”(201903);“烟草晒烤代换系的构建、评价及育种利用”(201902);贵州省科技厅基础研究计划项目“烟草单倍体诱导相关NtPLA基因的克隆及功能鉴定”(黔科合基础[2020]1Y067)。

摘　　要：【目的】提高烟草白粉病抗病育种效率,有效防止抗性丢失。【方法】通过烟草种质资源白粉病抗性鉴定获得抗源材料,利用F2和BCF1分离群体进行白粉病抗性遗传规律和分子标记连锁分析,开发与烟草白粉病抗性紧密连锁的分子标记。【结果】获得白粉病抗源材料2份,分别为台烟7号和KE1,其中台烟7号的白粉病抗性符合双基因隐性遗传规律。根据抗感亲本的NtMLO基因的差异开发了分子标记,利用分离群体证明该标记与白粉病抗性共分离,并应用于主栽品种的抗性回交改良。【结论】开发了与烟草Ntmlo白粉病抗性共分离的分子标记,可显著提高烟草白粉病抗病育种效率。[Objective]This study aims to improve the breeding efficiency of tobacco with powdery mildew resistance and prevent loss of resistance locus.[Methods]The powdery mildew resistance of tobacco germplasm resources was identified to obtain the resistant materials.The inheritance analyses of powdery mildew resistance were carried out in F2 and BC1F1 segregating populations.The molecular markers closely linked to tobacco powdery mildew resistance were developed according to the sequence difference of NtMLO genes.[Result]Two powdery mildew resistant materials were obtained.The powdery mildew resistance of Taiyan No.7 was inherited by two recessive genes.The molecular markers were proved to be co-segregated with powdery mildew resistance in segregation population,and have been applied to the backcross improvement of resistance of main cultivars.[Conclusion]Molecular markers cosegregated with Ntmlo resistance were developed,which could significantly improve the breeding efficiency of tobacco with powdery mildew resistance.

关 键 词：白粉病 分子标记 回交改良 资源鉴定 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]