肿瘤坏死因子-α对糖尿病大鼠肾脏肥大的影响  

Effects of tumor necrosis factor-αon renal hypertrophy in diabetic rats

作　　者：战翔宇 孙玉鸿[2] 陈瑞 赵阳 张东瑞 薛嵩 刘佳 隋洪玉[1] ZHAN Xiang-yu;SUN Yu-hong;CHEN Rui;ZHAO Yang;ZHANG Dong-rui;XUE Song;LIU Jia;SUI Hong-yu(School of Basic Medical Sciences,Jiamusi University,Jiamusi 154007,China;First Affiliated Hospital of Jiamusi University,Jiamusi 154003,China)

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2021年第5期10-11,18,共3页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省大学生创新创业训练计划项目,编号:202010222062;黑龙江省自然科学基金项目,编号:LH2019C065;黑龙江省卫生厅项目,编号:2018104。

摘　　要：目的:探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)对糖尿病大鼠肾脏肥大的影响。方法:成功制备糖尿病肾病大鼠模型后,采用蛋白免疫印迹等技术观察TNF-α对糖尿病大鼠肾脏肥大的影响。结果:1)与NC组相比,DM组空腹血糖及24h尿白蛋白排泄量明显升高(n=8,P<0.01);2)与NC组相比,DM组肾组织中TNF-α的蛋白表达明显升高(n=3,P<0.01);3)与NC组相比,DM组肾指数明显升高(n=8,P<0.01);与DM组相比,DM+TNFR:Fc组肾指数下降(n=8,P<0.05)。结论:糖尿病肾病时TNF-α水平增高,并促进糖尿病肾脏肥大的发生。Objective:To investigate the effect of tumor necrosis factor-α(TNF-α)on renal hypertrophy in diabetic rats.Methods:The diabetic nephropathy rats were divided into NC group,DM group and DM+TNF-αgroup.The effect of TNF-αon renal hypertrophy of diabetic rats was observed by western blot.Results:①Compared with NC group,fasting blood glucose and 24 h urinary albumin excretion in DM group were significantly increased(n=8,P<0.01).②Compared with NC group,TNF-αprotein expression in DM group was significantly increased(n=3,P<0.01).③Compared with NC group,renal index in DM group was significantly higher(n=8,P<0.01).Compared with DM group,the renal index in DM+TNF-αgroup was decreased(n=8,P<0.05).Conclusion:The increase of TNF-αin diabetic nephropathy promotes the occurrence of diabetic renal hypertrophy.

关 键 词：肿瘤坏死因子-Α 糖尿病 肾脏肥大 

分 类 号：R587.1[医药卫生—内分泌]