基于QuEChERS的UHPLC-MS/MS同时检测鸡蛋中19种农药残留量  

Simultaneous determination of 19 kinds of pesticide residues in eggs by UHPLC-MS/MS based on QuEChERS

作　　者：黄小波[1] 刘维平[1] HUANG Xiao-bo;LIU Wei-ping(Changsha Environmental Protection Vocational and Technical College,Changsha,Hunan 410004,China)

机构地区：[1]长沙环境保护职业技术学院,湖南长沙410004

出　　处：《食品与机械》2021年第10期56-60,共5页Food and Machinery

基　　金：湖南省自科基金科教联合项目(编号:2017JJ5060)。

摘　　要：目的:构建一种同时、快速、高效的检测鸡蛋中多种农残方法。方法:将QuEChERS与UHPLC-MS/MS结合用于鸡蛋中19种农药残留量的检测。结果:所建方法的检出限(LOD)和定量限(LOQ)分别为(0.39±0.66),(1.28±2.17)μg/kg;加标回收率为79.25%~102.14%,相对标准偏差均值(RSD)为9.34%~16.72%;除恶唑菌酮外,鸡蛋中其余18种农药的线性相关系数接近0.999。所测鸡蛋中鸡蛋中19种农残检出总频率为2.82%,农残问题主要涉及辛硫磷和毒死蜱,其检出频率分别为22%,15%,浓度范围分别为2.08~729.74,0.81~46.89μg/kg。结论:所建方法准确可靠,符合相关检测要求;湖北省境内鸡蛋中农残检出频次低、安全隐患较小。Objective:The aim is to establish a rapid,efficient and simultaneous detection method for various pesticide residues in eggs.Methods:QuEChERS combined with UHPLC-MS/MS was successfully used to detect 19 kinds of pesticide residues in eggs.Results:The limit of detection(LOD)and quantification(LOQ)of this method were(0.39±0.66)and(1.28±2.17)μg/kg,respectively.The recoveries of standard pesticides and relative standard deviations ranged from 79.25%to 102.14%and from 9.34%to 16.72%,respectively.Except for famoxadone,the correlation coefficients of linear equations for all pesticides were close to 0.999.The total frequency of 19 kinds of pesticides detected in eggs was 2.82%and the detection frequency of chlorpyrifos and phoxim were 22%and 15%,which maybe the potential safety issue for eggs.The quantity of them ranged from 2.08~729.74μg/kg and 0.81~46.89μg/kg,respectively.Conclusion:The proposed method is accurate,reliable,and meets the requirements for testing pesticides in eggs.The detection frequency of pesticides in eggs in Hubei province is low,indicating a low safety risk.

关 键 词：QUECHERS 鸡蛋 农药残留 超高效液相色谱—串联质谱 

分 类 号：O657.63[理学—分析化学] TS253.7[理学—化学]