基于分光吸收特性的辣椒叶片叶绿素含量测量方法研究  

Measurement of Chlorophyll Content in Pepper Leaves Based on the Absorption Characteristics of Spectrum

作　　者：郭宏亮[1,2] 黄帅 聂楚楚[3] 张悦 索玉祥 于合龙 王剑锋[3] GUO Hongliang;HUANG Shuai;NIE Chuchu;ZHANG Yue;SUO Yuxiang;YU Helong;WANG Jianfeng(Jilin Agricultural University,Changchun 130118;Jilin Precision Agriculture and Big Data Engineering Re-search Center,Changchun 130118;Jilin Academy of Vegetable and Flower Sciences,Changchun 130118,China)

机构地区：[1]吉林农业大学,长春130118 [2]吉林省精准农业与大数据工程研究中心,长春130118 [3]吉林省蔬菜花卉科学研究院,长春130118

出　　处：《东北农业科学》2021年第5期82-86,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省科技发展计划项目(20190301024NY);国家现代农业产业技术体系-特色蔬菜产业技术体系(CARS-24);吉林省科技计划项目(201910193137)。

摘　　要：为了无损、简单、快速、准确估测辣椒叶片叶绿素含量和组成,提升作物的精细管理水平。本文基于辣椒叶片对光的吸收特性,利用光谱透过率通过计算得到叶片的吸光度,使用四阶导数法在640~690 nm波段找到与叶片中叶绿素a和叶绿素b的吸收波峰:649、668、681 nm。参照化学法检测植物叶绿素含量和组成的吸光度法,使用多元线性回归分别建立叶绿素a和叶绿素b含量的检测模型并对比。其中以649、668、681 nm处吸光度建立的模型检测叶绿素a含量的结果最好,测试结果的相对误差(RE)为8.86%,决定系数(R^(2))为0.879;以649、681 nm处吸光度建立的模型检测叶绿素b含量的结果最好,测试结果的相对误差(RE)为9.84%,决定系数(R^(2))为0.878。In order to estimate the chlorophyll content and composition of pepper leaves without damage,simply,quickly,accurately and improve the level of crop fine management.In this paper,the absorbance of pepper leaves was calculated by spectral transmittance,the absorption peaks of chlorophyll a and chlorophyll b content in leaves were found at 640~690 nm by fourth order derivative method(649,668 and 681 nm).The detection models of chlorophyll a and chlorophyll b content were established and compared by using multiple linear regression.The model established by absorbance at 649,668 and 681 nm had the best results in predicting the content of chlorophyll a.the relative error(RE)of the test results was 8.86%,and the determination coefficient(R^(2))was 0.879.The model established by absorbance at 649 and 681 nm had the best results in predicting chlorophyll b content.The relative error(RE)of the test results was 9.84%,and the determination coefficient(R^(2))was 0.878.

关 键 词：辣椒 叶绿素检测 四阶导数 吸收光谱 

分 类 号：S641.3[农业科学—蔬菜学]