强直性脊柱炎患者T、B及NK淋巴细胞亚群检测的临床价值  

The clinical value of detection of T,B and NK lymphocyte subsets in patients with ankylosing spondylitis

作　　者：刘倩[1,2] 马亮[2] 岳德波[3] 刘辉[1,2] 姜永玮 徐英春[1] LIU Qian;MA Liang;YUE De-bo(Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academic Medical Science and Peking Union Medical College,Beijing 100730,China)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]中日友好医院检验科,北京100029 [3]中日友好医院骨科,北京100029

出　　处：《中日友好医院学报》2021年第5期276-279,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的:探讨强直性脊柱炎(AS)患者人类白细胞抗原B27(HLA-B27)基因和T、B及自然杀伤(NK)淋巴细胞亚群检测的临床价值。方法:收集中日友好医院2018年~2020年间确诊的121例AS患者以及120例健康体检人群外周血样本,检测HLA-B27基因及分型,流式细胞术检测其标记为T、B及NK的淋巴细胞亚群。结果:121例AS患者中HLA-B27基因阳性101例,其中HLA-B2704型58例、HLA-B2705型40例、HLA-B2702型1例、HLA-B2704*HLA-B2705复合杂合子2例。AS组HLA-B27基因阳性率明显高于健康对照组(83.5%vs2.5%,P<0.01)。HLA-B2705型AS组T%、Th%、Th/Ts明显高于HLA-B2704型AS患者(P<0.01),B1%、NK%显著低于HLA-B2704型AS患者(P<0.01)。101例HLA-B27基因阳性与20例HLA-B27基因阴性AS患者T、B及NK淋巴细胞亚群差异暂无统计学意义(P>0.05)。结论:AS患者淋巴细胞亚群分布异常,免疫功能紊乱,与HLA-B27基因亚型具有相关性。Objective:To explore the clinical significance of detection of human leukocyte antigen B27(HLAB27)gene,T,B and NK lymphocyte subsets in patients with ankylosing spondylitis(AS).Methods:Peripheral blood samples of 121 AS patients diagnosed in China-Japan Friendship Hospital from 2018 to 2020 and 120 healthy subjects were collected.Fluorescence quantitative PCR dye method was used to detect and typing HLA-B27 gene,and flow cytometry method was used to detect the lymphocyte subsets of T,B and NK.Results:One hundred and one of 121 AS patients were positive for HLA-B27 gene,of which 58 were HLA-B2704,40 were HLA-B2705,1 was HLA-B2702,and 2 were HLA-B2704*HLA-B2705 compound heterozygotes.The positive rate of HLA-B27 gene in AS group was significantly higher than that in healthy control group(83.5%vs2.5%,P<0.01).Compared with the HLA-B2704 type AS patients,the T%,Th% and Th/Ts were significantly higher(P<0.01),and the B1% and NK% were significantly lower(P<0.01)in the HLA-B2705 type AS patients.There was no significant difference in T,B,and NK lymphocyte subsets between the 101 HLA-B27 gene-positive and the 20 HLA-B27 gene-negative AS patients(P>0.05).Conclusion:The distribution of lymphocyte subsets in AS patients was abnormal,which was correlated with HLA-B27 gene subtypes,but has no correlation with HLA-B27 gene.

关 键 词：强直性脊柱炎 淋巴细胞亚群 人类白细胞抗原B27(HLA-B27) 基因分型 

分 类 号：R446.9[医药卫生—诊断学]