自身免疫型卵巢早衰模型的研究  

Study of a model of autoimmune premature ovarian failure

作　　者：王海丹 郭红玉 马蔚蓉 王琼 严士海 符蕊 WANG Haidan;GUO Hongyu;MA Weirong;WANG Qiong;YAN Shihai;FU Rui(Affiliated Hospital of Nanjing University of Chinese Medicine Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院江苏省中医院,南京210029

出　　处：《中国实验动物学报》2021年第5期563-569,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金青年基金项目(81804131)。

摘　　要：目的为了更好地建立自身型卵巢早衰模型,采用不同的造模时间和佐剂观察ZP3诱导的免疫型卵巢早衰模型建立的相关因素。方法将动情周期正常的B6AF1小鼠,随机分为正常组、模型A组、模型B组、模型C组、模型D组。造模结束后取小鼠血清检测性激素,取卵巢观察病理改变、透明带的变化、颗粒细胞的凋亡。结果除模型A组外,其余3组均表现出动情周期紊乱、高促性腺激素低雌激素、闭锁卵泡增多、透明带明显、卵巢凋亡细胞增多的现象,与各组相比模型C组和D组在闭锁卵泡数量、卵巢凋亡细胞和透明带的变化上更加明显。结论ZP3诱导可造成免疫型卵巢早衰,其中3次免疫的两组成模效果更好,成模率在80%,可作为今后建模的参考。Objective To improve a mouse model of autoimmune premature ovarian failure,different modeling times and adjuvant were used to determine factors related to induction of premature ovarian failure by zona pellucida glycoprotein 3(ZP3).Methods B6AF1 mice with regular estrous cycles were randomly divided into five groups:normal,model A,model B,model C and model D groups.At the end of the experiment,serum was collected to detect sex hormones,and ovaries were collected to observe pathological changes,including effects on the zona pellucida and apoptosis of ovarian cells.Results Compared with the normal group,the model B,C and D groups showed estrous cycle disorders,including high gonadotropin and low estrogen levels,increased follicle atresia,obvious zona pellucida and increased apoptosis of ovarian apoptotic cells.The model C and model D groups had more obvious changes in the number of atretic follicles,ovarian apoptotic cells and zona pellucida.Conclusions InZP3 induced autoimmune premature ovarian failure,the two-component model with three times immunization produced a stronger effect,and the modeling rate was 80%,which can be used as a reference for future modeling.

关 键 词：免疫型卵巢早衰 模型建立 透明带抗原 B6AF1小鼠 

分 类 号：Q95-33[生物学—动物学]