幽门螺杆菌的免疫学检测技术及其主要抗原表位  

Immunological detection of Helicobacter pylori and its main epitopes

作　　者：景荣先 刘昆梅[2] 徐铠琳 邢以文 薛满 汤锋[4] 郭乐[2] 张国林 JING Rongxian;LIU Kunmei;XU Kailin;XING Yiwen;XUE Man;TANG Feng;GUO Le;ZHANG Guolin(Department of Pharmacy,the Affiliated Suzhou Hospital of Nanjing Medical University(Suzhou Municipal Hospital),Suzhou 215000,China;Ningxia Medical University,Yinchuan 750021,China;Suzhou Institute for Drug Control,Suzhou 215000,China;Qinghai University,Xining 810001,China)

机构地区：[1]南京医科大学附属苏州医院(苏州市立医院)药学部,苏州215000 [2]宁夏医科大学,银川750021 [3]苏州市药品检验检测研究中心,苏州215000 [4]青海大学,西宁810001

出　　处：《西北药学杂志》2021年第5期844-848,共5页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:32070930);江苏省市场监督管理局科技项目(编号:KJ207561)。

摘　　要：目的了解幽门螺杆菌(Helicobacter pylori,H.pylori)的免疫学检测技术和主要抗原表位及其应用,为临床诊断及生物技术药物开发提供参考。方法查阅相关文献并结合本课题组的研究,分析H.pylori的免疫学检测技术和主要抗原表位及其应用。结果基于抗原-抗体反应的免疫层析技术是H.pylori的主要体外检测技术。H.pylori特异性抗原是基于免疫反应H.pylori检测的关键,也是相关疫苗开发的关键。尿素酶(Ure)、细胞毒素相关蛋白A/L(CagA/L)、空泡毒素相关蛋白A(VacA)、中性粒细胞激活蛋白(NAP)是H.pylori致病性关键表面抗原和病理学基础。结论基于多种抗原(或抗体)的组合表位同时具备了高灵敏度和高特异性的优点,其在H.pylori诊断和疫苗及抗体等生物技术药物开发方面具有广阔的前景。Objective To review the immunological detection technology of Helicobacter pylori(H.pylori)and its main epitopes in order to provide reference for clinical diagnosis and vaccine development.Methods Based on the related literature and the research of our research group,the immunological detection technology of H.pylori,the main epitopes and their applications were analyzed.Results Rapid and low-invasive analysis of H.pylori infection is very important for the diagnosis and treatment of H.pylori infected gastropathy.H.pylori specific antigen is the key to the detection technology of H.pylori based on immune response,and also the key to the development of H.pylori vaccine.Urease(Ure),cytotoxin associated gene A/L(CagA/L),vacuolating toxin associated protein(VacA)and neutrophil activating protein(NAP)are the key surface antigens and pathlogical basis of H.pylori pathogenicity.Conclusion The combination epitopes based on multiple antigens(or antibodies)have the advantages of high sensitivity and specificity,showing a broad prospect in the diagnosis of H.pylori and the development of biotechnological drugs such as vaccines and antibodies.

关 键 词：幽门螺杆菌(H.pylori) 尿素酶 细胞毒素相关蛋白A/L(Cag A/L) 空泡毒素相关蛋白A(VacA) 中性粒细胞激活蛋白(NAP) 

分 类 号：R978[医药卫生—药品]