HIV抗体免疫印迹实验不确定者的随访研究  

Follow-up study of cases with indeterminate HIV antibody immunoblotting test results

作　　者：殷方兰[1] 张永[1] 王嘉行[1] 倪胜男 吴杰[1] YIN Fang-lan;ZHANG Yong;WANG Jia-xing;NI Sheng-nan;WU Jie(Jiading Center for Disease Control and Prevention,ShangHai 201800,China)

机构地区：[1]上海市嘉定区疾病预防控制中心,上海201800

出　　处：《公共卫生与预防医学》2021年第6期95-98,共4页Journal of Public Health and Preventive Medicine

基　　金：上海市嘉定区卫健委科研项目(2018-KY-14)。

摘　　要：目的探讨HIV抗体不确定者分布及随访转归情况。方法回顾性分析2016—2019年嘉定区艾滋病确证实验室94例首次HIV抗体WB检测结果为HIV抗体不确定的病例及转归情况。结果HIV抗体不确定者主要来源于医疗机构就诊检测,占68.09%,其次是咨询检测和孕产期检查,分别占12.77%和10.64%。对48例HIV抗体不确定者进行随访检测,随访检测率51.06%,不同人群、带型随访率差异无统计学意义,其中25.00%在随访中抗体转阳,39.58%抗体转阴。咨询检测HIV抗体阳转率最高,为55.56%。94例HIV抗体不确定者共有14种WB带型,主要集中在p24、gpl60以及p24pl60,分别占54.26%、17.02%和9.57%。不同带型数量抗体转阳率差异有统计学意义(P<0.05),WB带型gp160gp41p24阳转率为100%,gp160p24、gp160、p24阳转率均为66.67%、37.50%和11.54%。结论HIV抗体不确定者主要来自医疗机构,随访复测率低,应加强对特定人群及WB带型样本的随访检测,尽早确证HIV感染状态。Objective To study the characteristics and follow-up results of 94 people with indeterminate Western blot(WB)results of HIV antibody.Methods A retrospective analysis was conducted on the characteristics and outcome of 94 patients with indeterminate WB results of HIV antibody from AIDS confirmation laboratories in Jiading District from 2016 to 2019.Results The 94 subjects camemainly from clinical diagnostictests,accounting for 68.09%of the total,followed by counseling tests and maternal examinations,accounting for 12.77%and 10.64%,respectively.Follow-up testing was performed on 48 patients with indeterminate HIV antibodies,with a follow-up detection rate of 51.06%.Among them,25.00%turned positive during the follow-up,and 39.58%turned negative.There was nosignificant difference in the follow-up rate between different population groups and WB band-types.The positive conversion rate of counseling test samples was the highest,accounting for 55.56%of all positive conversion cases.There was a total of 14 WB band types,mainly in p24,gpl60 and p24pl60,accounting for 54.26%,17.02%and 9.57%,respectively.There was asignificant difference in the positive conversion rate of antibodies with different patterns of WB bands.The positive conversion rate of WB band type gp160gp41p24 was 100%,while the positive rate of gp160p24,gp160,and p24 was 66.67%,37.5%and 11.54%,respectively.Conclusion The samples with indeterminate WB results of HIV antibodywere mainly from medical institutions,and the follow-up retest rate was low.Follow-up tests of specific populations and samples with specific patterns of WB bands should be strengthened to confirm HIV infection status as soon as possible.

关 键 词：艾滋病病毒 抗体不确定 免疫印迹 随防 

分 类 号：R181[医药卫生—流行病学]