青海高原不同种源西北小檗幼苗对低温胁迫的生理响应  

Physiological response of Berberis vernae seedlings from different provenances to low temperature stress in Qinghai plateau

作　　者：向前胜 张政[1] 张登山 王宁 赵越[1] 孙奎[1] 铁军[3] 李积鑫 更桑才仁 XIANG Qiansheng;ZHANG Zheng;ZHANG Dengshan;WANG Ning;ZHAO Yue;SUN Kui;TIE Jun;LI Jixin;GENGSANG Cairen(Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China;Shaoguan University,Shaoguan 512005,China;Natural Resources Bureau of Wulan County,Haixi Mongolian and Tibetan Autonomous Prefecture of Qinghai Province,Wulan 817100,China;Shangbeishan Forest Farm of Ledu District in Haidong,Haidong 810699,China;Natural Resources Bureau of Chengduo County in Yushu Tibetan Autonomous Prefecture,Chengduo 815000,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]韶关学院,广东韶关512005 [3]青海省海西蒙古族藏族自治州乌兰县自然资源局,青海乌兰817100 [4]青海省海东市乐都区上北山林场,青海海东810699 [5]青海省玉树藏族自治州称多县自然资源局,青海称多815000

出　　处：《青海大学学报》2021年第5期14-22,共9页Journal of Qinghai University

基　　金：青海省科学技术厅项目(2018-NK-A3,2019-HZ-814);国家自然科学基金项目(41961017,1661001)青海省科学技术厅三江源生态保护和建设二期(2018-S-1);韶关学院校级课题(432-9000419)。

摘　　要：为比较3处种源的西北小檗(Berberis vernae)的低温适应能力,文中以青海高原分布的3处种源的西北小檗150 d生幼苗为研究对象,测定不同种源枝条的低温半致死温度;同时人工控温进行低温胁迫,测定不同种源西北小檗幼苗叶片中MDA、可溶性蛋白和可溶性糖的含量以及SOD、POD和CAT活性的变化规律。结果显示:3处种源的西北小檗枝条低温半致死温度由低到高的顺序为玉树市下拉秀乡-21.55℃、西宁市大通县-16.83℃、海东市乐都区-15.78℃;低温条件下,玉树市下拉秀乡种源的西北小檗体内可溶性糖含量、可溶性蛋白含量、SOD活性、POD活性和CAT活性的增加量均要高于西宁市大通县种源和海东市乐都区种源,说明玉树市下拉秀乡种源的低温适应能力较其他两处种源强。本研究结果可为西北小檗耐寒种质的筛选、耐寒基因的挖掘以及高海拔城镇绿化树种的种源地选择提供理论依据。In order to compare the low temperature adaptability of Berberis vernae from three provenances,the 150-day seedlings of Berberis vernae from three provenances in Qinghai plateau are studied to determine the low-temperature semi-lethal temperature of branches from different provenances;at the same time,the content of MDA,soluble protein and soluble sugar are measured under the stress of low temperature as well as the activities of SOD,POD and CAT in the leaves of Berberis vernae seedlings from different provenances.The results show that the order of low-temperature semi-lethal temperature of Berberis vernae branches from three provenances is Yushu Xialaxiu-21.55℃,Xining Datong-16.83℃and Haidong Ledu-15.78℃.The increase of soluble sugar,soluble protein,SOD activity,POD activity and CAT activity in Berberis vernae from Yushu Xialaxiu are higher than those from Xining Datong and Haidong Ledu under low temperature,indicating that Yushu Xialaxiu has stronger adaptability to low temperature than the other two provenances.The results can provide a theoretical basis for the screening of cold resistant germplasm,the mining of cold resistant genes and selection of provenance of high-altitude urban greening tree species.

关 键 词：种源 西北小檗 低温半致死温度 抗寒性 

分 类 号：S567.19[农业科学—中草药栽培]