沼泽红假单胞菌蛋白Rhp-PSP对烟草花叶病毒的系统抗性诱导及互作蛋白筛选  

Systemic Resistance to Tobacco Mosaic Virus(TMV)Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein

作　　者：周寒梅 陈丽洁 翟忠英 张德咏 苏品[1,2] 刘勇 ZHOU Hanmei;CHEN Lijie;ZHAI Zhongying;ZHANG Deyong;SU Pin;LIU Yong(Longping Branch,Graduate School of Hunan University,Changsha 410125,China;Hunan Plant Protection Institute,Hunan Academy of Agricultural Science,Changsha 410125,China)

机构地区：[1]湖南大学研究生院隆平分院,长沙410125 [2]湖南省农业科学院植物保护研究所,长沙410125

出　　处：《中国生物防治学报》2021年第5期1073-1081,共9页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0200400);新疆农业科学院科技创新重点培育专项(xjkcpy-2020005);湖南省重点研发计划(2019NK2192)。

摘　　要：本文研究了蛋白Rhp-PSP诱导烟草抗TMV过程中相关防御酶的活性以及丙二醛(MDA)含量的变化,同时测定了抗病相关基因的表达情况。试验证明,蛋白Rhp-PSP处理烟草后,叶片中苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)的活性显著提高,但降低了MDA含量。同时基因PR-1、PR-3、PR-5、PDF1.2表达显著上调,证明了蛋白Rhp-PSP具有诱导烟草产生抗TMV的活性。为阐明诱导抗性机制,利用酵母双杂交系统从烟草c DNA文库进行互作蛋白的筛选并验证,结果表明GPI锚定蛋白、VAN3结合蛋白及叶绿素结合蛋白A与蛋白Rhp-PSP互作。The activity of defense enzymes,content of malondialdehyde(MDA)and expression level of resistance gene were determined in TMV inoculated tobacco plants induced by protein Rhp-PSP systemic resistance to TMV.The results showed that protein Rhp-PSP treatment increased the activities of phenylalanine ammonia-lyase(PAL),peroxidase(POD)and polyphenol oxidase(PPO)in TMV inoculated tobacco leaves,but decreased the content of MDA,and up-regulated the gene expression of PR-1,PR-3,PR-5 and PDF1.2 significantly.The above results indicated that protein Rhp-PSP of Rhodopseudomonas palustris could induce systemic resistance to TMV in tobacco.In order to understand the mechanism of induce systemic resistance,the interaction proteins with protein Rhp-PSP were screened in the tobacco cDNA library using yeast two-hybrid system.The result showed that the GPI anchoring protein,VAN3 binding protein,chlorophyll binding protein A may have interaction with Rhp-PSP.

关 键 词：沼泽红假单胞菌 防御酶 抗病相关基因 蛋白互作 

分 类 号：S476[农业科学—农业昆虫与害虫防治]